ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) Antikörper

Antigen: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1)

Synonyme: CLCS, MDR1, P-GP, PGY1, ABC20, CD243, GP170, MGC163296, Abcb1, Mdr1a, ABCB1, ABCB4, CMDR1, xemdr, p-gp

Klonalität: Monoklonal (C494)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN105306

Produktbeschreibung

Weitere Bezeichnung: p170 / p-Glycoprotein / MDR

Immunogen: BALB/C mice were injected with SDS-solublized plasma membranes of multidrug-resistant Chinese hamster ovary (CHRB30) and human (CEM/VLB500).

Format: Supernatant

Isotyp: IgG2a kappa  (Passende Sekundärantikörper)

Klon: C494

Spezifität: This antibody reacts with a 170 kD protein. Multi-drug resistance (MDR) associated with p-glycoprotein (p170) causes resistance to various drugs in a number of cancer patients. It exists as soluble and membrane associated form. Soluble p-glycoprotein is detected in extracellular fluids of cancer patients, such as malignant ascites and serum.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Adrenal. Cellular localization: Cell membrane

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as culture supernatant containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human adrenal stained with p170/MDR antibody

Publikationen

Publikationen: Bani, Brandi, Axiotis et al.: "Detection of P-glycoprotein on endothelial and endocrine cells of the human pancreatic islets by C 494 monoclonal antibody." in: Histochemistry, Vol. 98, Issue 4, pp. 207-9, 1993 (PubMed).

Georges, Bradley, Gariepy et al.: "Detection of P-glycoprotein isoforms by gene-specific monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 1, pp. 152-6, 1990 (PubMed).

Rao, Anthony, Piwnica-Worms: "MDR1 gene-specific monoclonal antibody C494 cross-reacts with pyruvate carboxylase." in: Cancer research, Vol. 54, Issue 6, pp. 1536-41, 1994 (PubMed).