Estrogen Receptor (ER) Antikörper

Antigen: Estrogen Receptor (ER)

Synonyme: LOC398733, LOC398734, ERa, ESR, Estr, ER[a], Estra, Nr3a1, ERalpha, AA420328, AU041214, ER-alpha, ESR1, NR3A1, eralpha, zfER[a], Errra, ERRalpha, ESRRA, err1, erra, esrral, erralpha, LOC100125507, ESTRRA, esrl1, nr3b1, MGC147343, ERRa, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, erb2, erb1, CERB, ESR2, ERB, erb, esrb, estrb, nr3a2, ERbeta, er-beta, esr-beta, ERBETA, er, ER-beta, ER[b], Estrb, ER[b]2, ER[b]a, zfER[b]2, zfER-beta2, LOC100125508, errb2, ER, Era, ESRA, DKFZp686N23123, 7B4, CD144, FLJ17376, ACTGP1, ACT1GP1, HY-psi-gamma-AC6, Esr, RNESTROR, ERALPHA, esr, esr1, nr3a1, AAC2, PNAT, XER, era, esra, xesr-1, xlERalpha1, xlERalpha2

Klonalität: Monoklonal (1D5)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN105035

Produktbeschreibung

Weitere Bezeichnung: Oestrogen Receptor

Immunogen: Recombinant human estrogen receptor protein.

Format: Supernatant

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: 1D5

Spezifität: This antibody is specific to estrogen receptor, which is associated with superior prognosis and a better response to endocrine therapy. The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm. However, on cryostat sections a positive staining of estrogen receptor in the nucleus as well as cytoplasm can be seen.

Synonyme: LOC398733, LOC398734, ERa, ESR, Estr, ER[a], Estra, Nr3a1, ERalpha, AA420328, AU041214, ER-alpha, ESR1, NR3A1, eralpha, zfER[a], Errra, ERRalpha, ESRRA, err1, erra, esrral, erralpha, LOC100125507, ESTRRA, esrl1, nr3b1, MGC147343, ERRa, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, erb2, erb1, CERB, ESR2, ERB, erb, esrb, estrb, nr3a2, ERbeta, er-beta, esr-beta, ERBETA, er, ER-beta, ER[b], Estrb, ER[b]2, ER[b]a, zfER[b]2, zfER-beta2, LOC100125508, errb2, ER, Era, ESRA, DKFZp686N23123, 7B4, CD144, FLJ17376, ACTGP1, ACT1GP1, HY-psi-gamma-AC6, Esr, RNESTROR, ERALPHA, esr, esr1, nr3a1, AAC2, PNAT, XER, era, esra, xesr-1, xlERalpha1, xlERalpha2

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25 to 1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear Other Applications: Western Blotting (1:50 to 1:100)

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Hormone, Rezeptoren

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human breast carcinoma stained with Estrogen Receptor antibody

Publikationen

Publikationen: Bur, Zimarowski, Schnitt et al.: "Estrogen receptor immunohistochemistry in carcinoma in situ of the breast." in: Cancer, Vol. 69, Issue 5, pp. 1174-81, 1992 (PubMed).

Graham, Jin, Lloyd: "Detection of estrogen receptor in paraffin-embedded sections of breast carcinoma by immunohistochemistry and in situ hybridization." in: The American journal of surgical pathology, Vol. 15, Issue 5, pp. 475-85, 1991 (PubMed).

Pertschuk, Kim, Nayer et al.: "Immunocytochemical estrogen and progestin receptor assays in breast cancer with monoclonal antibodies. Histopathologic, demographic, and biochemical correlations and relationship to endocrine response and survival." in: Cancer, Vol. 66, Issue 8, pp. 1663-70, 1990 (PubMed).

Kumar, Green, Stack et al.: "Functional domains of the human estrogen receptor." in: Cell, Vol. 51, Issue 6, pp. 941-51, 1988 (PubMed).