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STX1A Antikörper (N-Term)

STX1A Reaktivität: Human, Maus, Ratte, Affe, Schwein, Hamster, Xenopus laevis IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1049369
  • Target Alle STX1A Antikörper anzeigen
    STX1A (Syntaxin 1A (Brain) (STX1A))
    Bindungsspezifität
    • 17
    • 15
    • 12
    • 8
    • 6
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reaktivität
    • 73
    • 45
    • 29
    • 9
    • 9
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Affe, Schwein, Hamster, Xenopus laevis
    Wirt
    • 79
    • 8
    • 2
    • 1
    Kaninchen
    Klonalität
    • 81
    • 9
    Polyklonal
    Konjugat
    • 49
    • 7
    • 7
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser STX1A Antikörper ist unkonjugiert
    Applikation
    • 53
    • 38
    • 29
    • 16
    • 16
    • 14
    • 13
    • 13
    • 8
    • 6
    • 3
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human STX1A / Syntaxin 1A. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except STX2 (67 % ).
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Pig, Xenopus (100%) Panda, Dog, Bat, Bovine, Horse, Opossum, Turkey, Chicken (93%) Elephant (87%) Drosophila, Mosquito, Water flea, Nematode, Beetle (80%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 15 amino acid peptide from N-Terminus of human STX1A / Syntaxin 1A. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Pig, Xenopus (100%), Panda, Dog, Bat, Bovine, Horse, Opossum, Turkey, Chicken (93%), Elephant (87%), Drosophila, Mosquito, Water flea, Nematode, Beetle (80%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product STX1A Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (5 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    STX1A (Syntaxin 1A (Brain) (STX1A))
    Andere Bezeichnung
    STX1A / Syntaxin 1A (STX1A Produkte)
    Synonyme
    HPC-1 antikoerper, P35-1 antikoerper, STX1 antikoerper, SYN1A antikoerper, STX1A antikoerper, hpc-1 antikoerper, p35-1 antikoerper, stx1 antikoerper, sx1a antikoerper, syntaxin1a antikoerper, CG10716 antikoerper, CG18615 antikoerper, CG31136 antikoerper, CG5448 antikoerper, CT30033 antikoerper, Dm Syx1 antikoerper, DmSyx1A antikoerper, Dmel\\CG31136 antikoerper, SYX1A antikoerper, Syt1A antikoerper, Syx antikoerper, Syx-1A antikoerper, Syx1 antikoerper, Syx1a antikoerper, SyxA antikoerper, anon-EST:Gibbs4 antikoerper, anon-WO02059370.54 antikoerper, dSyx1 antikoerper, l(3)06737 antikoerper, synt antikoerper, syt-1A antikoerper, syx antikoerper, syx-1 antikoerper, syx-1A antikoerper, syx1 antikoerper, syx1A antikoerper, syntaxin 1A antikoerper, syntaxin 1A (brain) antikoerper, Syntaxin 1A antikoerper, syntaxin-1A antikoerper, STX1A antikoerper, Stx1a antikoerper, stx1a antikoerper, Syx1A antikoerper, EDI_005240 antikoerper, Tsp_07354 antikoerper
    Hintergrund
    Name/Gene ID: STX1A
    Family: Syntaxin/epimorphin

    Synonyms: STX1A, Syntaxin-1A, p35-1, SYN1a, Syntaxin 1A, Syntaxin 1A (brain), HPC-1, Neuron-specific antigen HPC-1, STX1
    Gen-ID
    6804
    Pathways
    Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
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