P2RY14 Antikörper (C-Term)

Produktnummer ABIN1049214

Produktdetails anti-P2RY14 Antikörper

Target: P2RY14 (Purinergic Receptor P2Y, G-Protein Coupled, 14 (P2RY14))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser P2RY14 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Marke: IHC-plus™

Spezifität: Human P2RY14 / P2Y14. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human (100%) Monkey (95%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from C-terminus of human P2RY14 / P2Y14. Percent identity with other species by BLAST analysis: Human (100%), Monkey (95%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: ICC (5 - 10 μg/mL), IHC, IHC-P (4 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu P2RY14

Target: P2RY14 (Purinergic Receptor P2Y, G-Protein Coupled, 14 (P2RY14))

Andere Bezeichnung: P2RY14 / GPR105 (P2RY14 Produkte)

Synonyme: BPR105 antikoerper, GPR105 antikoerper, P2Y14 antikoerper, A330108O13Rik antikoerper, Gpr105 antikoerper, purinergic receptor P2Y, G-protein coupled, 14 antikoerper, purinergic receptor P2Y14 antikoerper, P2RY14 antikoerper, P2ry14 antikoerper

Hintergrund: Name/Gene ID: P2RY14
Subfamily: Sugar sensing
Family: GPCR

Synonyms: P2RY14, G-protein coupled receptor 105, p2Y purinoceptor 14, p2Y(14) receptor, p2Y14 receptor, UDP-glucose receptor, p2Y14, VTR 15-20 receptor, G protein-coupled receptor 105, GPR105, KIAA0001, p2RY14

Gen-ID: 9934