P2RY12 Antikörper (Extracellular Domain)

Produktnummer ABIN1049208

Produktdetails anti-P2RY12 Antikörper

Target: P2RY12 (Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12))

Bindungsspezifität: Extracellular Domain

Reaktivität: Human, Hund, Affe, Kaninchen, Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser P2RY12 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human P2RY12 / P2Y12. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CACNA1F (56 % ).

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Horse, Rabbit (100%) Bat, Pig, Platypus (94%) Mouse, Hamster, Bovine, Opossum (88%) Rat, Elephant (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 2nd extracellular domain of human P2RY12 / P2Y12. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Horse, Rabbit (100%), Bat, Pig, Platypus (94%), Mouse, Hamster, Bovine, Opossum (88%), Rat, Elephant (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 - 20 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu P2RY12

Target: P2RY12 (Purinergic Receptor P2Y, G-Protein Coupled, 12 (P2RY12))

Andere Bezeichnung: P2RY12 / P2Y12 (P2RY12 Produkte)

Synonyme: 2900079B22Rik antikoerper, 4921504D23Rik antikoerper, P2Y12 antikoerper, ADPG-R antikoerper, BDPLT8 antikoerper, HORK3 antikoerper, P2T(AC) antikoerper, P2Y(12)R antikoerper, P2Y(AC) antikoerper, P2Y(ADP) antikoerper, P2Y(cyc) antikoerper, SP1999 antikoerper, P2y12 antikoerper, purinergic receptor P2Y, G-protein coupled 12 antikoerper, purinergic receptor P2Y12 antikoerper, P2ry12 antikoerper, P2RY12 antikoerper

Hintergrund: Name/Gene ID: P2RY12
Subfamily: Purinergic
Family: GPCR

Synonyms: P2RY12, ADP-glucose receptor, ADPG-R, p2Y(12)R, p2Y(ADP), p2T(AC), p2Y12, Platelet ADP receptor, SP1999, p2RY12, p2Y purinoceptor 12, Purinergic receptor P2RY12, BDPLT8, Gi-coupled ADP receptor HORK3, HORK3, p2Y(AC), p2Y(cyc), p2Y12 platelet ADP receptor

Gen-ID: 64805