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P2RX7 Antikörper (Internal Region)

P2RX7 Reaktivität: Human, Affe IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1049197
  • Target Alle P2RX7 Antikörper anzeigen
    P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))
    Bindungsspezifität
    • 15
    • 11
    • 7
    • 5
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 58
    • 40
    • 19
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Human, Affe
    Wirt
    • 57
    • 2
    • 2
    • 1
    Kaninchen
    Klonalität
    • 58
    • 4
    Polyklonal
    Konjugat
    • 28
    • 7
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser P2RX7 Antikörper ist unkonjugiert
    Applikation
    • 48
    • 26
    • 23
    • 13
    • 13
    • 12
    • 10
    • 9
    • 6
    • 4
    • 3
    • 3
    • 2
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human P2RX7 / P2X7. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except DDX59 (56 % ).
    Homologie
    Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%) Marmoset, Pig (88%) Mouse, Horse (81%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 16 amino acid peptide from internal region of human P2RX7 / P2X7. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%), Marmoset, Pig (88%), Mouse, Horse (81%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product P2RX7 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (10 - 20 μg/mL)
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))
    Andere Bezeichnung
    P2RX7 / P2X7 (P2RX7 Produkte)
    Synonyme
    p2xr7 antikoerper, P2RX7 antikoerper, P2X7 antikoerper, AI467586 antikoerper, P2X(7) antikoerper, P2X7R antikoerper, p2x7 antikoerper, purinergic receptor P2X 7 antikoerper, purinergic receptor P2X, ligand-gated ion channel, 7 antikoerper, P2X purinoceptor 7 antikoerper, P2RX7 antikoerper, p2rx7 antikoerper, LOC100458463 antikoerper, P2rx7 antikoerper
    Hintergrund
    Name/Gene ID: P2RX7
    Subfamily: P2X receptor
    Family: Ion Channel

    Synonyms: P2RX7, ATP receptor, p2X purinoceptor 7, p2X receptor 7, p2X7 purinoceptor, p2Z receptor, Purinergic receptor, p2RX7, p2X7, p2X7 receptor
    Gen-ID
    5027
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis
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