DPP6 Antikörper (Internal Region)

Produktnummer ABIN1048510

Produktdetails anti-DPP6 Antikörper

Target: DPP6 (Dipeptidyl-Peptidase 6 (DPP6))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DPP6 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human DPP6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Gibbon, Monkey (94%) Horse (89%) Panda, Bovine (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from near the center of human DPP6. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon, Monkey (94%), Horse (89%), Panda, Bovine (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu DPP6

Target: DPP6 (Dipeptidyl-Peptidase 6 (DPP6))

Andere Bezeichnung: DPP6 / Dipeptidylpeptidase 6 (DPP6 Produkte)

Synonyme: dppx antikoerper, DPPX antikoerper, VF2 antikoerper, DPP VI antikoerper, dpp6 antikoerper, si:ch211-198f16.1 antikoerper, B930011P16Rik antikoerper, D5Buc3 antikoerper, D5Buc4 antikoerper, D5Buc5 antikoerper, Dpp-6 antikoerper, Gm1377 antikoerper, In(5)6H-p antikoerper, Peplb antikoerper, Rw antikoerper, dipeptidyl peptidase like 6 antikoerper, dipeptidyl-peptidase 6 antikoerper, dipeptidyl-peptidase 6b antikoerper, dipeptidylpeptidase 6 antikoerper, DPP6 antikoerper, dpp6 antikoerper, BCAN_A2221 antikoerper, BSUIS_A2016 antikoerper, Bsph_2289 antikoerper, BMEA_A2239 antikoerper, dpp6b antikoerper, Dpp6 antikoerper

Hintergrund: Name/Gene ID: DPP6
Subfamily: Serine S9B
Family: Protease

Synonyms: DPP6, Dipeptidylpeptidase VI, Dipeptidyl peptidase 6, Dipeptidyl peptidase VI, Dipeptidyl-peptidase 6, Dipeptidylpeptidase 6, DPP VI, DPPX, VF2, Dipeptidyl aminopeptidase-like protein 6

Gen-ID: 1804