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beta Catenin Antikörper (C-Term)

CATNB Reaktivität: Human, Maus, Ratte, Rind (Kuh), Zebrafisch (Danio rerio), Xenopus laevis WB, ELISA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1043907
  • Target Alle beta Catenin (CATNB) Antikörper anzeigen
    beta Catenin (CATNB) (Catenin, beta (CATNB))
    Bindungsspezifität
    • 43
    • 27
    • 21
    • 21
    • 20
    • 18
    • 17
    • 17
    • 17
    • 16
    • 16
    • 15
    • 9
    • 8
    • 7
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 332
    • 248
    • 226
    • 29
    • 23
    • 22
    • 18
    • 18
    • 5
    • 5
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Rind (Kuh), Zebrafisch (Danio rerio), Xenopus laevis
    Wirt
    • 272
    • 59
    • 4
    • 2
    • 1
    Kaninchen
    Klonalität
    • 267
    • 71
    Polyklonal
    Konjugat
    • 124
    • 22
    • 16
    • 15
    • 15
    • 15
    • 15
    • 15
    • 11
    • 10
    • 9
    • 9
    • 9
    • 9
    • 9
    • 8
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    Dieser beta Catenin Antikörper ist unkonjugiert
    Applikation
    • 208
    • 158
    • 120
    • 118
    • 74
    • 63
    • 62
    • 49
    • 36
    • 31
    • 22
    • 8
    • 7
    • 4
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Spezifität
    Beta catenin antibody is directed against catenin beta-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with catenin beta-1 protein from human, mouse, rat, bovine, Xenopus laevis, and Danio rerio at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
    Produktmerkmale
    Beta-catenin 1 (or ß-catenin 1) is a protein that is encoded by the CTNNB1 gene. ß-catenin 1 is a subunit of the cadherin protein complex and has been implicated as an integral component in the Wnt signaling pathway. This pathway plays a key role in the regulation of cellular processes involved in development, differentiation, and adult tissue homeostasis. In the presence of Wnt ligand, ß-catenin 1 is not ubiquitinated and accumulates in the nucleus, where it associates with T-cell factor (TCF) family members to regulate target gene expression in many developmental and adult tissues. Recruitment of b-catenin 1 to Wnt response element (WRE) chromatin converts TCFs from transcriptional repressors to activators. ß-catenin 1 is also involved in the regulation of cell adhesion. It acts as a negative regulator of centrosome cohesion. Aberrant Wnt/ß-catenin signaling is widely implicated in cancer, bone disorders, kidney and intestinal cell disorders and other disease states. ß-catenin 1 is located in the cytoplasm when it is unstabilized or bound to CDH1. Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of ß-catenin 1 is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. It further co-localizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells.
    Sterilität
    Sterile filtered
    Immunogen
    beta catenin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to catenin beta-1 C-terminus.
    Immunogen Type: Peptide
    Isotyp
    IgG
  • Applikationshinweise
    beta catenin antibody has been tested for use in ELISA, western blotting, and ISH. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85.5 kDa in size corresponding to catenin beta-1 protein by western blotting in the appropriate cell lysate or extract.
    Kommentare

    Gene Name: ctnnb1

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029816 (Western Blotting)
    'Independent Validation' Siegel
    by
    ADS Biosystems Inc
    No.
    #029816
    Datum
    18.09.2014
    Antigen
    Chargennummer
    27397
    Validierte Anwendung
    Western Blotting
    Positivkontrolle
    MCF-7 cells
    Negativkontrolle
    SK-BR-3 cells
    Bewertung
    A strong specific band was observed in the positive control at the expected size (~85.5 kDa) that is not observed in the negative control.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antigen: Beta Catenin
    • Catalog number: ABIN1043907
    • Lot number: 27397
    • Dilution: 1:1,000
    Sekundärantikörper
    • Antibody: IRDye 680LT Goat Anti-Rabbit
    • Lot number: C30725-01
    • Dilution: 1:10,000
    Full Protocol
    • Lysates were mixed with NuPAGE® LDS Sample Buffer (Life Technologies NP0007) and NuPAGE® Sample Reducing Agent (Life Technologies NP0004) and denatured for 5 minutes at 90ºC.
    • 40 μg of each lysate was electrophoresed on a Bolt 4-12% Bis-Tris Gel (Life Technologies BG04120BOX) and run in Bolt MOPS SDS Running Buffer (Life Technologies B0001) at 160 volts for 1 hour.
    • Odyssey Western Protein Standard (LI-COR #928-40000) was run as a molecular weight standard.
    • PVDF membrane was activated with methanol.
    • Protein samples were transferred to activated PVDF membrane in a wet Bolt Transfer Apparatus (Life Technologies B1000) at room temperature for 1 hour at 20 volts (started at 230mA, ended at 110mA).
    • The membrane was blocked in x LI-COR Odyssey WB block solution for 1 hour at room temperature.
    • The membrane was incubated with the primary antibody diluted 1:1000 in x LI-COR Odyssey WB block solution incubated 2 hours at room temperature.
    • The membrane was washed 4 x 5 minutes in 1 x PBS-T (PBS solution with 0.1% Tween 20).
    • The membrane was incubated with IRDye® 800CW Goat anti-Mouse Secondary Antibody (Red) and IRDye 680LT Goat Anti-Rabbit Secondary Antibody (Green) from LI-COR (#827-11081, Lot #C30725-01), both 1:10,000 dilutions. Incubation was performed at room temperature for 45 minutes.
    • The membrane was washed 4 x 5 minutes in 1 x PBS-T (PBS solution with 0.1% Tween 20).
    • Proteins were detected using Odyssey machine scanning with green channel for loading control and red channel for potential LPL band.
    Anmerkungen
    - No experimental challenges noted.
  • Format
    Liquid
    Konzentration
    1.15 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Haltbarkeit
    12 months
  • Huge, Sandbothe, Schröder, Stalke, Eilers, Schäffer, Schlegelberger, Illig, Vajen, Skawran: "Wnt status-dependent oncogenic role of BCL9 and BCL9L in hepatocellular carcinoma." in: Hepatology international, Vol. 14, Issue 3, pp. 373-384, (2020) (PubMed).

    Mo, Chew, Maher, Bellipanni, Weinberg, Gottardi: "The terminal region of beta-catenin promotes stability by shielding the Armadillo repeats from the axin-scaffold destruction complex." in: The Journal of biological chemistry, Vol. 284, Issue 41, pp. 28222-31, (2009) (PubMed).

  • Target
    beta Catenin (CATNB) (Catenin, beta (CATNB))
    Andere Bezeichnung
    beta Catenin (CATNB Produkte)
    Synonyme
    Bfc antikoerper, Catnb antikoerper, Mesc antikoerper, CTNNB antikoerper, MRD19 antikoerper, armadillo antikoerper, ctnnb antikoerper, id:ibd2058 antikoerper, wu:fb73e10 antikoerper, wu:fi81c06 antikoerper, wu:fk25h01 antikoerper, beta-catenin antikoerper, ctnnb1 antikoerper, CHBCAT antikoerper, catenin (cadherin associated protein), beta 1 antikoerper, catenin beta 1 antikoerper, catenin (cadherin-associated protein), beta 1 antikoerper, catenin beta 1 L homeolog antikoerper, Ctnnb1 antikoerper, CTNNB1 antikoerper, ctnnb1 antikoerper, ctnnb1.L antikoerper
    Hintergrund
    Beta-catenin 1 (or ß-catenin 1) is a protein that is encoded by the CTNNB1 gene. ß-catenin 1 is a subunit of the cadherin protein complex and has been implicated as an integral component in the Wnt signaling pathway. This pathway plays a key role in the regulation of cellular processes involved in development, differentiation, and adult tissue homeostasis. In the presence of Wnt ligand, ß-catenin 1 is not ubiquitinated and accumulates in the nucleus, where it associates with T-cell factor (TCF) family members to regulate target gene expression in many developmental and adult tissues. Recruitment of b-catenin 1 to Wnt response element (WRE) chromatin converts TCFs from transcriptional repressors to activators. ß-catenin 1 is also involved in the regulation of cell adhesion. It acts as a negative regulator of centrosome cohesion. Aberrant Wnt/ß-catenin signaling is widely implicated in cancer, bone disorders, kidney and intestinal cell disorders and other disease states. ß-catenin 1 is located in the cytoplasm when it is unstabilized or bound to CDH1. Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of ß-catenin 1 is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. It further co-localizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells.
    Synonyms: Catenin beta-1, beta catenin, ß-catenin-1, ß-catenin, CTNNB1, CTNNB, beta catenin antibody, anti-beta catenin
    NCBI Accession
    NP_001091679
    UniProt
    Q9WU82
    Pathways
    Peptide Hormone Metabolism
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