His Tag Antikörper

Produktnummer ABIN1043868

Produktdetails anti-His Tag Antikörper

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Sequenz: HHHHHH

Spezifität: This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Produktmerkmale: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

Sterilität: Sterile filtered

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-His Tag Antikörper (ABIN1043868)

Mart Nez-Cruz, Romero, Hierrezuelo, Thon, de Vicente, P Rez-Garc A: "Effectors with chitinase activity (EWCAs), a family of conserved, secreted fungal chitinases that suppress chitin-triggered immunity." in: The Plant cell, Vol. 33, Issue 4, pp. 1319-1340, (2021) (PubMed).

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Koh, Cao, Mangos, Tardi, Dande, Lee, Samelko, Altintas, Schmitz, Lee, Mukherjee, Peev, Cimbaluk, Reiser, Hahm: "Nonimmune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist." in: The Journal of clinical investigation, Vol. 129, Issue 4, pp. 1713-1726, (2019) (PubMed).

Vela-Corcía, Romero, de Vicente, Pérez-García: "Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance." in: Scientific reports, Vol. 8, Issue 1, pp. 7161, (2019) (PubMed).

Lecona, Rodriguez-Acebes, Specks, Lopez-Contreras, Ruppen, Murga, Muñoz, Mendez, Fernandez-Capetillo: "USP7 is a SUMO deubiquitinase essential for DNA replication." in: Nature structural & molecular biology, Vol. 23, Issue 4, pp. 270-7, (2016) (PubMed).

Lucchetti, Caligiuri, Toffoli, Giordano, Rizzolio: "The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor α in breast cancer." in: PLoS ONE, Vol. 8, Issue 2, pp. e55355, (2013) (PubMed).

La Montagna, Caligiuri, Maranta, Lucchetti, Esposito, Paggi, Toffoli, Rizzolio, Giordano: "Androgen receptor serine 81 mediates Pin1 interaction and activity." in: Cell cycle (Georgetown, Tex.), Vol. 11, Issue 18, pp. 3415-20, (2013) (PubMed).

Bradley, Chan, Hagood: "Effect of the GPI anchor of human Thy-1 on antibody recognition and function." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 93, Issue 3, pp. 365-74, (2013) (PubMed).

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García-López, Sánchez-Amaya, Halm, Astola, Prat et al.: "Bone morphogenetic protein 15 and growth differentiation factor 9 expression in the ovary of European sea bass (Dicentrarchus labrax): cellular localization, developmental profiles, and response to ..." in: General and comparative endocrinology, Vol. 174, Issue 3, pp. 326-34, (2012) (PubMed).

Sorrentino, Scheer, Santos, Reits, Bleijlevens, Zelcer: "Distinct functional domains contribute to degradation of the low density lipoprotein receptor (LDLR) by the E3 ubiquitin ligase inducible Degrader of the LDLR (IDOL)." in: The Journal of biological chemistry, Vol. 286, Issue 34, pp. 30190-9, (2011) (PubMed).

Niederkofler, Baeriswyl, Ott, Stoeckli: "Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance." in: Development (Cambridge, England), Vol. 137, Issue 3, pp. 427-35, (2010) (PubMed).

Monegal, Ami, Martinelli, Huang, Aliprandi, Capasso, Francavilla, Ossolengo, de Marco: "Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library." in: Protein engineering, design & selection : PEDS, Vol. 22, Issue 4, pp. 273-80, (2009) (PubMed).

Yi, Ba, Zhang, Ho, Chen: "Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain." in: Journal of virology, Vol. 79, Issue 18, pp. 11638-46, (2005) (PubMed).

Details zu His Tag

Target: His Tag

Abstract: His Tag Produkte

Substanzklasse: Tag

Hintergrund: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.