TIMP4 Antikörper

Produktnummer ABIN1043743

Produktdetails anti-TIMP4 Antikörper

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TIMP4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Spezifität: Anti-TIMP4 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. TIMP4 antibody is specific for human TIMP4 protein. This antibody reacts with human TIMP4 in tissue sections of formalin-fixed, paraffin-embedded In ELISAs and Native-PAGE, this antibody does not cross-react with recombinant human TIMP-1, -2 or -3. A BLAST analysis was used to suggest cross-reactivity with TIMP4 from human and mouse sources based on the immunizing sequence. Cross-reactivity with TIMP-4 from other sources has not been determined.

Produktmerkmale: Tissue inhibitor of metalloproteinase-4 (TIMP-4) is member of the TIMP family of inhibitors of matrix metalloproteinase (MMP) activities. In addition, TIMPs have non-MMP associated functions, including roles in growth promotion, apoptosis, and angiogenesis. All four members of the TIMP gene family form a similar secondary structure due to the formation of six highly conserved disulfide bonds. It has been reported that some TIMPs, TIMP4 included, can bind a tetraspanin in the plasma membrane, initiating an intra-cellular signaling cascade. Elevated levels of TIMP4 have been associated with breast cancer progression and heart disease.

Sterilität: Sterile filtered

Immunogen: Anti-TIMP4 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with full legnth human TIMP4 protein followed by hybridoma development.
Immunogen Type: RecombinantProtein

Klon: 9:4-7

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Anti-TIMP4 antibody has been tested for use in ELISA, Native - Western, and IHC (P). SDS-PAGE - Western Blot not useful for endogenous detection. Specific conditions for reactivity should be optimized by the end user.

Kommentare:

Gene Name: TIMP4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-TIMP4 Antikörper (ABIN1043743)

Donover, Wojciechowski, Thirumaran, Zemba-Palko, Prendergast, Wallon: "Development of a monoclonal antibody that specifically detects tissue inhibitor of metalloproteinase-4 (TIMP-4) in formalin-fixed, paraffin-embedded human tissues." in: Journal of cellular biochemistry, Vol. 110, Issue 5, pp. 1255-61, (2010) (PubMed).

Details zu TIMP4

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Andere Bezeichnung: TIMP4 (TIMP4 Produkte)

Synonyme: TIMP4 antikoerper, TIMP-4 antikoerper, timp4 antikoerper, TIMP metallopeptidase inhibitor 4 antikoerper, TIMP metallopeptidase inhibitor 4, tandem duplicate 3 antikoerper, tissue inhibitor of metalloproteinase 4 antikoerper, TIMP4 antikoerper, timp4.3 antikoerper, Timp4 antikoerper

Hintergrund: Tissue inhibitor of metalloproteinase-4 (TIMP-4) is member of the TIMP family of inhibitors of matrix metalloproteinase (MMP) activities. In addition, TIMPs have non-MMP associated functions, including roles in growth promotion, apoptosis, and angiogenesis. All four members of the TIMP gene family form a similar secondary structure due to the formation of six highly conserved disulfide bonds. It has been reported that some TIMPs, TIMP4 included, can bind a tetraspanin in the plasma membrane, initiating an intra-cellular signaling cascade. Elevated levels of TIMP4 have been associated with breast cancer progression and heart disease.
Synonyms: Tissue inhibitor of metalloproteinase-4, Metalloproteinase inhibitor 4, TIMP-4, TIMP 4, TIMP4 antibody, anti-TIMP4 antibody

Gen-ID: 7079

NCBI Accession: NM_003256

UniProt: Q99727