Lactate Dehydrogenase A (LDHA) Antikörper

Antigen: Lactate Dehydrogenase A (LDHA)

Synonyme: LDH1, LDHM, GSD11, PIG19, LDHA, LDH-M, Ldh1, Ldhm, l7R2, ldhbb, ldha-B, MGC83980, ldh1, ldhm, gsd11, ldh-m, pig19, MGC53139, MGC76171, MGC90004, ldha1, ldhab, LDH-A, LDHC, DKFZp469J2231

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Rind (Kuh), Human, Maus, Ratte (Rattus), Schaf

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN103412

Produktbeschreibung

Weitere Bezeichnung: Lactate Dehydrogenase (LDH)

Swiss-Prot: P13491

Immunogen: This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle. Protein Sequence: Rabbit Lactate Dehydrogenase A chain (muscle), 332 aa, predicted MW 36.6kDa 1 maalkdqlih nllkeehvpq nkitvvgvga vgmacaisil mkdladelal vdvmedklkg 61 emmdlqhgsl flrtpkivsg kdysvtansk lviitagarq qegesrlnlv qrnvnifkfi 121 ipnvvkysph ckllvvsnpv diltyvawki sgfpknrvig sgcnldsarf rylmgerlgv 181 halschgwil gehgdssvpv wsgmnvagvs lktlhpelgt dadkeqwkqv hkqvvdsaye 241 viklkgyttw aiglsvadla esimknlrrv hpistmlkgl ygikedvfls vpcvlgqngi 301 sdvvkvtlts eeeahlkksa dtlwgiqkel qf

Format: Lyophilized

Beschreibung: "Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a ""flipped pattern"") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization."

Spezifität: This product was prepared from monospecific antiserum by delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species.

Anwendungen

Applikationshinweise: This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract.

Konzentration: 80 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

Forschungsgebiet: Enzyme, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis shows antibodies-online's Anti-Lactate Dehydrogenase antibody detects LDH in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). ~30 ug was loaded per lane onto a 10% gel for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the protein. The blot was incubated with a 1:1,000 dilution of the antibody in 5% milk in TBST at room temperature followed by detection using standard procedures. Personal communication Kuldeep Patel.

Publikationen

Publikationen: He, Borland, Zhu et al.: "Effect of ligand activation of peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) in human lung cancer cell lines." in: Toxicology, Vol. 254, Issue 1-2, pp. 112-7, 2008 (PubMed).

Shiflett, Mauna, Chipman et al.: "Appetitive Pavlovian conditioned stimuli increase CREB phosphorylation in the nucleus accumbens." in: Neurobiology of learning and memory, Vol. 92, Issue 3, pp. 451-4, 2009 (PubMed).

Miyata, Hosoi, Mukai et al.: "In vitro import of peroxisome-targeting signal type 2 (PTS2) receptor Pex7p into peroxisomes." in: Biochimica et biophysica acta, Vol. 1793, Issue 5, pp. 860-70, 2009 (PubMed).

Liu, Zhang, Yin et al.: "alpha-Synuclein is differentially expressed in mitochondria from different rat brain regions and dose-dependently down-regulates complex I activity." in: Neuroscience letters, Vol. 454, Issue 3, pp. 187-92, 2009 (PubMed).

Foreman, Sharma, Amin et al.: "Ligand activation of peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) inhibits cell growth in a mouse mammary gland cancer cell line." in: Cancer letters, 2009 (PubMed).