KU (p80) Antikörper

Antigen: KU

Epitope: p80

Klonalität: Monoklonal (S10B1)

Wirt: Maus

Reaktivität: Human, Affe

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunpräzipitation (IP), Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN103406

Produktbeschreibung

Weitere Bezeichnung: KU (p80)

Immunogen: BALB/C mice were injected with human placental extract (PSE1-PL).

Format: Ig-fraction

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: S10B1

Spezifität: This antibody reacts with a 80 kD protein known as Ku (p80). Ku autoantigen is a heterodimer of p70 kD (p70) and 80 kD (p80) proteins. The p70/p80 dimer is important for the function of a 460 kD DNA-dependant protein kinas that phosphorylates certain transcription factors, including sp1, Oct-1, p53 and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation and apoptosis. This antibody cross reacts with monkey but does not cross react with cow, rabbit and mouse.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear Other Applications: Immunoprecipitation, Immunofluorescence, Western Blotting

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Knuth, Gunderson, Thompson et al.: "Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter." in: The Journal of biological chemistry, Vol. 265, Issue 29, pp. 17911-20, 1990 (PubMed).

Ajmani, Satoh, Reap et al.: "Absence of autoantigen Ku in mature human neutrophils and human promyelocytic leukemia line (HL-60) cells and lymphocytes undergoing apoptosis." in: The Journal of experimental medicine, Vol. 181, Issue 6, pp. 2049-58, 1995 (PubMed).