JAK3 Antikörper

Antigen: JAK3

Klonalität: Monoklonal (A1-14-16)

Wirt: Maus

Reaktivität: Human, Maus

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN103337

Produktbeschreibung

Immunogen: BALB/C mice were immunized with recombinant protein encoding C-terminus corresponding to amino acid 1076

Format: Purified

Isotyp: IgG2a kappa  (Passende Sekundärantikörper)

Klon: A1-14-16

Spezifität: This antibody with a protein of 115 kD. JAK3 is a member of the Janus family of protein tyrosine kinases along with JAK1, JAK2 and Tyk2. JAK3 is activated in response to IL-3 and IL-4 in T cells and myeloid cells. JAK3 activation requires the serine-rich membrane-proximal region of the IL-2 receptor beta-chain. Expression of JAK3 is restricted to NK and T cells. This antibody cross reacts with mouse.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Colon carcinoma. Cellular localization: Cell membrane, cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Signaltransduktion, Zytokine, Rezeptoren, Kinasen/Phosphatasen

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.