Interleukin 2 Receptor Type 2 Antikörper

Antigen: Interleukin 2 Receptor Type 2

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunzytochemie (ICC), Neutralisierung (Neut), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN103285

Produktbeschreibung

Weitere Bezeichnung: Interleukin-2 Receptor Type II (IL-1RII)

Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-1R type II.

Format: Liquid (sterile filtered)

Spezifität: This antiserum has been heated to 56oC for 30 minutes. The antiserum is directed against the extracellular domain of the human IL-1R type II, but precipitates the intact receptor expressed on human cells. In general, this antibody also detects primate IL-1RII. The antibody does not recognize human IL-1, IL-1Ra or IL-1R type I. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type II (also called soluble IL-1R type II) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid. Endotoxin Content: <10 pg/ ul by LAL method.

Anwendungen

Applikationshinweise: Recommended Dilutions: This product has been assayed for the ability to immunoprecipitate antigen. A dilution range of 1:400 to 1:800 is suggested for this immunoassay. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (code ABIN101990) and TMB as a substrate. A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against soluble (extracellular) IL-1RII using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code ABIN101990) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:1,000 to 1:2000 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8,000 is suggested for this immunoassay. Optimal titers for other applications should be determined by the researcher. Note: This antiserum against anti-Human IL-1 Receptor Type II (IL-1RII) has been tested for use in ELISA, radioimmunoassays and immunoprecipitation. Reactivity in other immunoassays is unknown. IL-1RII has been termed p68 or CD121 in some laboratories. IL-1RII has been shown to bind IL-1, IL-1 alpha and IL-1beta.

Konzentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening.

Forschungsgebiet: Zytokine, Rezeptoren, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunoblot using antibodies-online's anti-IL- 1RII antibody. This blot shows detection of an IL-1RII-GST fusion protein (~500 ng, lane 1, green, 800 nm channel). Minimal reactivity is observed against GST (data not shown). Protein was resolved on a 4-20% Tris- Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was for 2 hrs at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye