CD73 Antikörper

Produktnummer ABIN1027668

Produktdetails anti-CD73 Antikörper

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD73 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytometry by Time of Flight (CyTOF)

Spezifität: The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells and endothelial cells.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: pre-B leukemia cells

Klon: AD2

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CD73 Antikörper (ABIN1027668)

Semenov, Koestenbauer, Riegel, Zech, Zimmermann, Zisch, Malek: "Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation." in: American journal of obstetrics and gynecology, Vol. 202, Issue 2, pp. 193.e1-193.e13, (2010) (PubMed).

Mokry, Soukup, Micuda, Karbanova, Visek, Brcakova, Suchanek, Bouchal, Vokurkova, Ivancakova: "Telomere attrition occurs during ex vivo expansion of human dental pulp stem cells." in: Journal of biomedicine & biotechnology, Vol. 2010, pp. 673513, (2010) (PubMed).

Paquet-Fifield, Schlüter, Li, Aitken, Gangatirkar, Blashki, Koelmeyer, Pouliot, Palatsides, Ellis, Brouard, Zannettino, Saunders, Thompson, Li, Kaur: "A role for pericytes as microenvironmental regulators of human skin tissue regeneration." in: The Journal of clinical investigation, Vol. 119, Issue 9, pp. 2795-806, (2009) (PubMed).

Rodriguez, Paquet, Yang, Erle: "Differential gene expression by integrin beta 7+ and beta 7- memory T helper cells." in: BMC immunology, Vol. 5, pp. 13, (2004) (PubMed).

Hashikawa, Takedachi, Terakura, Saho, Yamada, Thompson, Shimabukuro, Murakami: "Involvement of CD73 (ecto-5'-nucleotidase) in adenosine generation by human gingival fibroblasts." in: Journal of dental research, Vol. 82, Issue 11, pp. 888-92, (2003) (PubMed).

Nemoto, Tada, Shimauchi: "Disruption of CD40/CD40 ligand interaction with cleavage of CD40 on human gingival fibroblasts by human leukocyte elastase resulting in down-regulation of chemokine production." in: Journal of leukocyte biology, Vol. 72, Issue 3, pp. 538-45, (2002) (PubMed).

Borrione, Peola, Mariani, Besostri, Mallone, Malavasi, Pileri, Massaia: "CD38 stimulation lowers the activation threshold and enhances the alloreactivity of cord blood T cells by activating the phosphatidylinositol 3-kinase pathway and inducing CD73 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 6238-46, (1999) (PubMed).

Details zu CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Andere Bezeichnung: CD73 (NT5E Produkte)

Synonyme: CALJA antikoerper, CD73 antikoerper, E5NT antikoerper, NT antikoerper, NT5 antikoerper, NTE antikoerper, eN antikoerper, eNT antikoerper, zgc:63784 antikoerper, NT5E antikoerper, MGC114869 antikoerper, PSPTO2277 antikoerper, 2210401F01Rik antikoerper, AI447961 antikoerper, Nt5 antikoerper, 5'-nucleotidase ecto antikoerper, 5'-nucleotidase, ecto (CD73) antikoerper, 5'-nucleotidase antikoerper, 5' nucleotidase, ecto antikoerper, NT5E antikoerper, nt5e antikoerper, nt5e.S antikoerper, PSPTO_2277 antikoerper, CBU_1233 antikoerper, MCA1322 antikoerper, Nt5e antikoerper

Hintergrund: 5'-nucleotidase ecto,CD73 (ecto-5´-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein.,ecto-5´nucleotidase, NT5E, E5NT, CALJA, NTE

Gen-ID: 4907

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process