Maus anti-Human Immunoglobulin lambda (IgL) Antikörper

Antigen: Immunoglobulin lambda (IgL)

Klonalität: Monoklonal (HP6054)

Wirt: Maus

Reaktivität: Human

Konjugat: Unkonjugiert

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN102518

Produktbeschreibung

Weitere Bezeichnung: Immunoglobulin Lambda

Immunogen: Purified human IgG myeloma proteins covalently linked to polyaminostyrene micro beads.

Format: Supernatant (prediluted)

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: HP6054

Spezifität: This antibody stains B cell follicles in human lymphoid tissues. The mantle zone shows a mosaic pattern of labeling while the germinal centers show a coarse meshwork pattern of staining. Lambda light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkins lymphomas. This antibody does not react with rat.

Anwendungen

Applikationshinweise: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cytoplasm

Konzentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Jefferis, Reimer, Skvaril et al.: "Evaluation of monoclonal antibodies having specificity for human IgG subclasses: results of the 2nd IUIS/WHO collaborative study." in: Immunology letters, Vol. 31, Issue 2, pp. 143-68, 1992 (PubMed).

Reimer, Phillips, Aloisio et al.: "Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes." in: Hybridoma, Vol. 3, Issue 3, pp. 263-75, 1985 (PubMed).

Black, McDougal, Holman et al.: "Cross-reactivity of 75 monoclonal antibodies to human immunoglobulin with sera of non-human primates." in: Immunology letters, Vol. 37, Issue 2-3, pp. 207-13, 1994 (PubMed).