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Kaninchen anti-Maus IgG1 (Heavy Chain) Antikörper (Biotin) - Preadsorbed

ELISA, WB, IHC Polyclonal IgG Biotin
Produktnummer ABIN102335
  • Target Alle IgG1 Produkte
    IgG1
    Bindungsspezifität
    • 41
    • 26
    • 17
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy Chain
    Reaktivität
    • 89
    • 25
    • 23
    • 7
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 65
    • 40
    • 34
    • 13
    • 10
    • 6
    • 1
    Kaninchen
    Klonalität
    • 97
    • 59
    Polyklonal
    Konjugat
    • 45
    • 18
    • 18
    • 15
    • 11
    • 8
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Applikation
    • 76
    • 56
    • 55
    • 41
    • 32
    • 21
    • 18
    • 12
    • 11
    • 10
    • 10
    • 9
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    Spezifität
    IgG1
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Aufreinigung
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Mouse IgG1 heavy chain

    Isotyp
    IgG
    Labeling Ratio
    10-20
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:500 - 1:3,000

    Application Note: Mouse IgG1 secondary antibody conjugated to Biotin is available in a variety of formats. Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.

    ELISA Dilution: 1:250,000

    Western Blot Dilution: 1:1,000 - 1:5,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Aliquot to Avoid repeated freezing and thawing.
    Lagerung
    RT,4 °C,-20 °C
    Haltbarkeit
    12 months
  • Target
    IgG1
    Abstract
    IgG1 Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: Rabbit Anti-Mouse IgG1 (Gamma 1 chain) Antibody biotin Conjugation, Rabbit Anti-Mouse IgG1 BAC Conjugated Antibody

    Background: Anti-Mouse IgG1 Biotin Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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