Ziege anti-Ratte (Rattus) IgG (Heavy & Light Chain) Antikörper (Biotin)

Details zu Produkt Nr. ABIN102119, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Heavy & Light Chain
4680
2537
1879
1066
1006
860
298
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reaktivität
Ratte (Rattus)
2850
2735
2296
1674
1433
788
613
442
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Wirt
Ziege
6704
4831
1795
891
609
388
91
55
52
21
8
5
5
5
2
1
1
Klonalität
Polyklonal
Konjugat
Biotin
2236
1872
1737
1356
767
682
519
297
264
223
222
200
156
138
128
110
79
75
69
55
51
46
43
40
38
36
34
34
32
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
15
15
15
15
15
15
14
12
12
12
10
10
9
9
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Rat IgG whole molecule
Isotyp IgG
Spezifität IgG (H&L)
Produktmerkmale RAT IgG Biotin Conjugated antibody detects rat immunoglobulin G.
Concentration Definition: by UV absorbance at 280 nm
Hintergrund Immunoglobuoin G is a molecule of about 150 kDa composed of four peptide chains. Each IgG contains two identical γ heavy chains of about 51 kDa and two identical light chains of about 26 kDa, thus a tetrameric quaternary structure. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape. Each end of the fork contains an identical antigen binding site. The Fc regions of IgGs bear a highly conserved N-glycosylation site.
Molekulargewicht 150 kDa
Forschungsgebiet Immunology, Secondary Antibodies
Applikationshinweise Anti-RAT IgG Biotin Conjugated antibody is suitable for immunoassays where specificity to the Rat immunoglobulin heavy and or light chain regions is desired. Anti-Rat antibody has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:500,000 is suggested for this product. Optimal concentrations in immunoassays should be determined by the researcher.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Restore with deionized water (or equivalent)
Konzentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Aliquot to Avoid repeated freezing and thawing.
Bilder des Herstellers
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Biotin) (ABIN102119) Western Blot of Goat anti-Rat IgG (H&L) Antibody Biotin Conjugated. Lane 1: Rat IgG. ...
Allgemeine Veröffentlichungen Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).