Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (Texas Red (TR)) - Preadsorbed

Produktnummer ABIN101770

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Texas Red (TR)

Applikation: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Spezifität: IgG (H&L)

Produktmerkmale: Mouse IgG (H&L) Antibody Texas Red™ Conjugated Pre-Adsorbed antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG whole molecule

Isotyp: IgG

Labeling Ratio: 3.9

Anwendungsinformationen

Applikationshinweise:

Application Note: Mouse IgG (H&L) Antibody Texas Red™ Conjugated Pre-Adsorbed antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Kommentare:

Texas Red™ is a registered trademark of Molecular Probes Inc.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Product is photosensitive and should be protected from light.

Lagerung: RT,4 °C,-20 °C

Haltbarkeit: 12 months

Referenzen

Chaillou, Zhang, McCarthy: "Expression of Muscle-Specific Ribosomal Protein L3-Like Impairs Myotube Growth." in: Journal of cellular physiology, Vol. 231, Issue 9, pp. 1894-902, (2017) (PubMed).

Fry, Lee, Mula, Kirby, Jackson, Liu, Yang, Mendias, Dupont-Versteegden, McCarthy, Peterson: "Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia." in: Nature medicine, Vol. 21, Issue 1, pp. 76-80, (2015) (PubMed).

Liu, Fry, Mula, Jackson, Lee, Peterson, Yang: "Automated fiber-type-specific cross-sectional area assessment and myonuclei counting in skeletal muscle." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 115, Issue 11, pp. 1714-24, (2014) (PubMed).

Fry, Lee, Jackson, Kirby, Stasko, Liu, Dupont-Versteegden, McCarthy, Peterson: "Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 4, pp. 1654-65, (2014) (PubMed).

Mula, Lee, Liu, Yang, Peterson: "Automated image analysis of skeletal muscle fiber cross-sectional area." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 114, Issue 1, pp. 148-55, (2013) (PubMed).

McCarthy, Mula, Miyazaki, Erfani, Garrison, Farooqui, Srikuea, Lawson, Grimes, Keller, Van Zant, Campbell, Esser, Dupont-Versteegden, Peterson: "Effective fiber hypertrophy in satellite cell-depleted skeletal muscle." in: Development (Cambridge, England), Vol. 138, Issue 17, pp. 3657-66, (2011) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Goat Anti Mouse IgG (H&L) Texas Red™ Conjugated Antibody Pre-Adsorbed, Goat Anti-Mouse IgG Antibody Texas Red™ Conjugation

Background: Anti-Mouse IgG Texas Red Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.