Esel anti-Ziege IgG (Heavy & Light Chain) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN101169

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Ziege

Wirt: Esel

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: IgG (H&L)

Kreuzreaktivität: Ziege

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Goat IgG (H&L) was produced by repeated immunization with goat IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotyp: IgG

Labeling Ratio: 10-20

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Antibody Anti-Goat IgG (H&L) is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:200,000 - 1:250,000

Western Blot Dilution: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Aliquot to Avoid repeated freezing and thawing.

Lagerung: RT,4 °C,-20 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: donkey anti-Goat IgG Antibody biotin Conjugation, donkey anti-Goat IgG biotin Conjugated Antibody

Background: Anti-Goat IgG Biotin Antibody generated in donkey detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.