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Ziege anti-Huhn IgG (Heavy & Light Chain) Antikörper (Biotin)

ELISA, IHC, WB Polyclonal IgG Biotin
Produktnummer ABIN101015
  • Target Alle IgG Produkte
    IgG
    Bindungsspezifität
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reaktivität
    • 695
    • 672
    • 561
    • 458
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 121
    • 98
    • 97
    • 95
    • 92
    • 62
    • 47
    • 38
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Huhn
    Wirt
    • 1729
    • 1474
    • 539
    • 206
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Ziege
    Klonalität
    • 3360
    • 167
    • 1
    Polyklonal
    Konjugat
    • 557
    • 409
    • 406
    • 367
    • 298
    • 276
    • 228
    • 121
    • 99
    • 67
    • 66
    • 62
    • 62
    • 52
    • 48
    • 47
    • 45
    • 44
    • 44
    • 41
    • 39
    • 34
    • 33
    • 33
    • 31
    • 31
    • 30
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    Biotin
    Applikation
    • 1839
    • 1800
    • 1791
    • 1500
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 52
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Spezifität
    IgG (H&L)
    Kreuzreaktivität
    Huhn
    Kreuzreaktivität (Details)
    Pre-Adsorbed
    Produktmerkmale
    Anti-Chicken IgG (H&L) Antibody generated in Goat detects specifically Chicken IgG (H&L). This secondary Anti-Chicken IgG (H&L) Antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Chicken IgG (H&L) Antibody is ideal for researchers in Immunology, Cancer, and Microbiology research.
    Concentration Definition: by UV absorbance at 280 nm
    Aufreinigung
    Anti-Chicken IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Chicken IgG and Chicken Serum.   No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.
    Immunogen

    Immunogen: Chicken IgG whole molecule

    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Chicken IgG (H&L) Antibody is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Aliquot to Avoid repeated freezing and thawing.
    Lagerung
    RT,4 °C,-20 °C
    Haltbarkeit
    12 months
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: Goat anti-Chicken IgG Antibody Biotin Conjugation, Goat-a-Chicken Biotin conjugated, Chicken IgG (H&L) Antibody in goat, Goat anti-Chicken IgG Biotin conjugated Secondary Antibody

    Background: Anti-Chicken IgG Biotin Antibody generated in goat detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Anti-Chicken IgG (H&L) Antibody is ideal for researchers in Immunology, Cancer, and Microbiology research.

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