HPV16 Antikörper

Antigen: HPV16

Klonalität: Monoklonal (CAMVIR-1)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN100574

Produktbeschreibung

Weitere Bezeichnung: HPV 16

Immunogen: BALB/C mice were immunized with recombinant HPV-16 L1 protein.

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: CAMVIR-1

Spezifität: This antibody stains with HPV type 16. L1 protein is a major capsid protein of HPV type 16. HPV 6 and 11 have been associated with relatively benign diseases such as genital warts but type 16 and 18 are strongly associated with cervical, vaginal and vulvar malignancies.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: HPV type 16 infected cervix. Cellular localization: Nuclear

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Virologie

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: McLean, Churcher, Meinke et al.: "Production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus." in: Journal of clinical pathology, Vol. 43, Issue 6, pp. 488-92, 1990 (PubMed).

Browne, Churcher, Stanley et al.: "Analysis of the L1 gene product of human papillomavirus type 16 by expression in a vaccinia virus recombinant." in: The Journal of general virology, Vol. 69 ( Pt 6), pp. 1263-73, 1988 (PubMed).