Neuregulin 1 (NRG1) Antikörper

Antigen: Neuregulin 1 (NRG1)

Synonyme: GGF, HGL, HRG, NDF, ARIA, GGF2, HRG1, HRGA, SMDF, HRG4, DKFZp779N0541, DKFZp779N1944, Hgl, GGFII, HRGalpha, 6030402G23Rik, D230005F13Rik, NRG1, MGC154949, nrg1-A, nrg1, NRG-1, wu:fm70e01, DKFZp459A1838

Klonalität: Monoklonal (7D5)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN100397

Produktbeschreibung

Weitere Bezeichnung: Heregulin/NDF/GGF/Neuregulin

Immunogen: BALB/C mice were injected with a recombinant extracellular domain of rat NDF protein.

Format: Ig-fraction

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: 7D5

Spezifität: This antibody reacts with a 44 kD protein known as Heregulin or neu differentiation factor (NDF). Heregulin binds to e-erbB-3 and c-erbB-4 receptors on mammary and neuronal cells with low and high affinities, respectively. HRG/NDF exists in several isoforms, which are classified in two groups, alpha and beta that differ in their EGF- like domain and in receptor binding affinity. This antibody cross reacts with mouse and rat.

Synonyme: GGF, HGL, HRG, NDF, ARIA, GGF2, HRG1, HRGA, SMDF, HRG4, DKFZp779N0541, DKFZp779N1944, Hgl, GGFII, HRGalpha, 6030402G23Rik, D230005F13Rik, NRG1, MGC154949, nrg1-A, nrg1, NRG-1, wu:fm70e01, DKFZp459A1838

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Prostate carcinoma. Cellular localization: Cytoplasmic, intercellular spaces Other Applications: Immunoprecipitation, Western Blotting (1:100-500).

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Wachstumsfaktoren

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human prostate carcinoma stained with Heregulin antibody

Publikationen

Publikationen: Peles, Bacus, Koski et al.: "Isolation of the neu/HER-2 stimulatory ligand: a 44 kd glycoprotein that induces differentiation of mammary tumor cells." in: Cell, Vol. 69, Issue 1, pp. 205-16, 1992 (PubMed).

Holmes, Sliwkowski, Akita et al.: "Identification of heregulin, a specific activator of p185erbB2." in: Science (New York, N.Y.), Vol. 256, Issue 5060, pp. 1205-10, 1992 (PubMed).

Lupu, Cardillo, Harris et al.: "Interaction between erbB-receptors and heregulin in breast cancer tumor progression and drug resistance." in: Seminars in cancer biology, Vol. 6, Issue 3, pp. 135-45, 1996 (PubMed).