Hairy Cell Leukaemia Antikörper

Antigen: Hairy Cell Leukaemia

Klonalität: Monoklonal (DBA-44)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN100197

Produktbeschreibung

Immunogen: BALB/C mice were injected with Deau cell line established from a large cell lymphoma.

Format: Supernatant (prediluted)

Isotyp: IgM kappa  (Passende Sekundärantikörper)

Klon: DBA-44

Spezifität: This antibody reacts with over 97% of hairy cell leukemia as well as about 35% of high grade B cell lymphomas. It reacts with B lymphocytes, cells of mantle zone and immunoblasts outside the lymphoid tissues.

Anwendungen

Applikationshinweise: Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane, cytoplasm

Konzentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Krebs

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: al Saati, Caspar, Brousset et al.: "Production of anti-B monoclonal antibodies (DBB.42, DBA.44, DNA.7, and DND.53) reactive on paraffin-embedded tissues with a new B-lymphoma cell line grafted into athymic nude mice." in: Blood, Vol. 74, Issue 7, pp. 2476-85, 1989 (PubMed).