GFP Antikörper (Green Fluorescent Protein) (AA 246)

Details for Product anti-GFP Antibody No. ABIN100085, Anbieter: Anmelden zum Anzeigen
Antigen
  • green fluorescent protein
  • gfp
Epitop
AA 246
42
31
28
17
5
4
3
3
2
1
1
1
1
1
1
Reaktivität
Aequorea victoria
638
16
5
5
2
2
Wirt
Ziege
340
171
79
40
16
9
9
2
Klonalität
Polyklonal
Konjugat
Dieser GFP Antikörper ist unkonjugiert
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31
28
14
12
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12
10
9
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3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
536
353
202
156
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96
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73
25
15
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12
8
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2
2
1
1
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Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant
Isotyp IgG
Produktmerkmale Concentration Definition: by UV absorbance at 280 nm
Sterilität Sterile filtered
Andere Bezeichnung GFP
Hintergrund Goat Anti-GFP is ideal for western blotting, ELISA, Immunohistochemistry and IP. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
UniProt P42212
Forschungsgebiet Tags/Labels
Applikationshinweise Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on western blots. Researchers should determine optimal titers for applications.
Kommentare

Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) (AA 246) antibody (ABIN100085) antibodies-online's polyclonal anti-GFP antibody at a 1:1,000 dilution detects tau-GF...
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) (AA 246) antibody (ABIN100085) Western Blot of Rabbit anti-GFP antibody. Lane 1: HeLa cells. Lane 2: mock transfecte...
 image for anti-Green Fluorescent Protein (GFP) (AA 246) antibody (ABIN100085) anti-Green Fluorescent Protein (GFP) (AA 246) antibody (Image 3)
Produkt verwendet in: Chotard, Skorobogata, Sylvain, Shrivastava, Rocheleau: "TBC-2 Is Required for Embryonic Yolk Protein Storage and Larval Survival during L1 Diapause in Caenorhabditis elegans." in: PLoS ONE, Vol. 5, Issue 12, pp. e15662, 2011 (PubMed).

Brett, Renault, Rafalski, Webb, Brunet: "The microRNA cluster miR-106b~25 regulates adult neural stem/progenitor cell proliferation and neuronal differentiation." in: Aging, Vol. 3, Issue 2, pp. 108-24, 2011 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (IHC) (1:500, Probematerial (Species): Mouse (Murine)).

Xu, Leinwand, Dell, Fried-Cassorla, Raper: "The calmodulin-stimulated adenylate cyclase ADCY8 sets the sensitivity of zebrafish retinal axons to midline repellents and is required for normal midline crossing." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 21, pp. 7423-33, 2010 (PubMed).

Taura, Miura, Iwaisako, Osterreicher, Kodama, Penz-Osterreicher, Brenner: "Hepatocytes do not undergo epithelial-mesenchymal transition in liver fibrosis in mice." in: Hepatology (Baltimore, Md.), Vol. 51, Issue 3, pp. 1027-36, 2010 (PubMed).

La: "A dual promoter lentiviral vector for the in vivo evaluation of gene therapeutic approaches to axon regeneration after spinal cord injury." in: Gene therapy, Vol. 17, Issue 5, pp. 577-91, 2010 (PubMed).

Maher-Laporte, Berthiaume, Moreau, Julien, Lapointe, Mourez, DesGroseillers: "Molecular composition of staufen2-containing ribonucleoproteins in embryonic rat brain." in: PLoS ONE, Vol. 5, Issue 6, pp. e11350, 2010 (PubMed).

Hilgen, von Maltzahn, Willecke, Weiler, Dedek: "Subcellular distribution of connexin45 in OFF bipolar cells of the mouse retina." in: The Journal of comparative neurology, Vol. 519, Issue 3, pp. 433-50, 2010 (PubMed).

Puthussery, Gayet-Primo, Taylor: "Localization of the calcium-binding protein secretagogin in cone bipolar cells of the mammalian retina." in: The Journal of comparative neurology, Vol. 518, Issue 4, pp. 513-25, 2009 (PubMed).

Justice, Yuan, Sawchenko, Vale: "Type 1 corticotropin-releasing factor receptor expression reported in BAC transgenic mice: implications for reconciling ligand-receptor mismatch in the central corticotropin-releasing factor system." in: The Journal of comparative neurology, Vol. 511, Issue 4, pp. 479-96, 2008 (PubMed).

Kofidis, de Bruin, Yamane, Tanaka, Lebl, Swijnenburg, Weissman, Robbins: "Stimulation of paracrine pathways with growth factors enhances embryonic stem cell engraftment and host-specific differentiation in the heart after ischemic myocardial injury." in: Circulation, Vol. 111, Issue 19, pp. 2486-93, 2005 (PubMed).

Balsam, Wagers, Christensen, Kofidis, Weissman, Robbins: "Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium." in: Nature, Vol. 428, Issue 6983, pp. 668-73, 2004 (PubMed).

Saxena, Saffery, Wong, Kalitsis, Choo: "Centromere proteins Cenpa, Cenpb, and Bub3 interact with poly(ADP-ribose) polymerase-1 protein and are poly(ADP-ribosyl)ated." in: The Journal of biological chemistry, Vol. 277, Issue 30, pp. 26921-6, 2002 (PubMed).

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