Human MICA ELISA Kit

Recommended MICA Product (geliefert von: Anmelden zum Anzeigen )

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MHC Class I Polypeptide-Related Sequence A (MICA) ELISA Kits
  • Ica
  • mICA
  • FLJ60820
  • PERB11.1
  • MGC111087
  • MIC-A
  • RIKEN cDNA 1300017J02 gene
  • MHC class I polypeptide-related sequence A
  • 1300017J02Rik
  • MICA
Epitop
AA 24-307
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
21
2
1
1
1
1
1
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
62.5-4000 pg/mL
Untere Nachweisgrenze
62.5 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Bilder

Produktnummer ABIN411398
$ 400.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
10.750587 ABIN625337 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 20-10000 pg/mL 20 pg/mL Anmelden zum Anzeigen 3
10.750587 ABIN2708319 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
10.750587 ABIN2114578 Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum Anmelden zum Anzeigen
1 ABIN3032960 Competition ELISA Cell Culture Supernatant, Plasma, Serum 13.7-10000 pg/mL 13.7 pg/mL Anmelden zum Anzeigen
1 ABIN5594946 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN2684970 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Lysate 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen 3
1 ABIN1446129 Sandwich ELISA Cell Culture Supernatant, Cell Lysate 123 pg/mL Anmelden zum Anzeigen
1 ABIN993844 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate 250-5000 pg/mL 250 pg/mL Anmelden zum Anzeigen
1 ABIN5525102 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN5656877 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 13.7 pg/mL - 10000 pg/mL 13.7 pg/mL Anmelden zum Anzeigen
1 ABIN1112748 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN2042593 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN2684971 Sandwich ELISA Cell Lysate, Tissue Lysate 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN2684969 Sandwich ELISA Cell Culture Supernatant, Cell Lysate 156-5000 pg/mL 156 pg/mL Anmelden zum Anzeigen
1 ABIN1846998 Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN585310 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN2116226 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Anmelden zum Anzeigen
1 ABIN2949483 78-5000 pg/mL 78 pg/mL Anmelden zum Anzeigen
1 ABIN2648784 Anmelden zum Anzeigen
1 ABIN5073291 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Anmelden zum Anzeigen

General

Antigen MHC Class I Polypeptide-Related Sequence A (MICA) ELISA Kits
Epitop AA 24-307
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(21), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methodentyp Sandwich ELISA
Detektionsbereich 62.5-4000 pg/mL
Untere Nachweisgrenze 62.5 pg/mL
Applikation ELISA
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails MICA ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Referenzen für MICA Kit (ABIN411398) Bilder
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MICA
Marke PicoKine™
Proben Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum
Nachweismethode Colorimetric
Analytische Methode Quantitative
Spezifität Expression system for standard: NSO
Immunogen sequence: E24-W307
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: E24-W307

Antigendetails

Produktdetails MICA ELISA Kit Anwendungsinformationen Handhabung Referenzen für MICA Kit (ABIN411398) Bilder zurück nach oben
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Andere Bezeichnung MICA (MICA ELISA Kit Abstract)
Hintergrund

Protein Function: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T- cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. .

Background: MHC class I polypeptide-related sequence A is a protein that in humans is encoded by the MICA gene.1 The MICA gene encodes a 383-amino acid polypeptide with a predicted mass of 43 kD.The MICA and MICB genes occur in a 200-kb region spanning the TNFA and TNFB cluster at 6p21.3.2 MICA and the closely related MICB were recognized by intestinal epithelial T cells expressing diverse V-delta-1 gamma/delta TCRs.3 The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is further distinguished by its unusual exon-intron organization and preferential expression in fibroblasts and epithelial cells. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA and other members of this family may have been selected for specialized functions that are either ancient or derived from those of typical MHC class I genes, in analogy to some of the nonclassic mouse H-2 genes.

Synonyms: MHC class I polypeptide-related sequence A,MIC-A,MICA ,PERB11.1 ,

Full Gene Name: MHC class I polypeptide-related sequence A

Cellular Localisation: Cell membrane, Single- pass type I membrane protein . Cytoplasm . Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum..
Gen-ID 4276
UniProt Q29983
Forschungsgebiet Immunology, Innate Immunity, Adaptive Immunity
Pathways Activation of Innate immune Response, Transition Metal Ion Homeostasis

Anwendungsinformationen

Produktdetails MICA ELISA Kit Antigendetails Handhabung Referenzen für MICA Kit (ABIN411398) Bilder zurück nach oben
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Sequence similarities: Belongs to the MHC class I family. MIC subfamily.

Tissue Specificity: Widely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles. Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T-cells. .

Plattentyp Pre-coated
Protokoll human MICA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MICA has been precoated onto 96-well plates. Standards(NSO, E24-W307) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MICA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MICA amount of sample captured in plate.
Testdurchführung Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human MICA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue lysates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MICA standard solution and each sample be measured in duplicate.
Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 447, Standard deviation: 22.8, CV(%): 5.1
  • Sample 2: n=16, Mean(pg/ml): 1327, Standard deviation: 75.64, CV(%): 5.7
  • Sample 3: n=16, Mean(pg/ml): 2443, Standard deviation: 151.5, CV(%): 6.2,
  • Sample 1: n=24, Mean(pg/ml): 526, Standard deviation: 34.2, CV(%): 6.5
  • Sample 2: n=24, Mean(pg/ml): 1648, Standard deviation: 115.4, CV(%): 7
  • Sample 3: n=24, Mean(pg/ml): 2756, Standard deviation: 209.5, CV(%): 7.6
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails MICA ELISA Kit Antigendetails Anwendungsinformationen Referenzen für MICA Kit (ABIN411398) Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months

Referenzen für MICA Kit (ABIN411398)

Produktdetails MICA ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Groh, Steinle, Bauer, Spies: "Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells." in: Science (New York, N.Y.), Vol. 279, Issue 5357, pp. 1737-40, 1998

Nalabolu, Shukla, Nallur, Parimoo, Weissman: "Genes in a 220-kb region spanning the TNF cluster in human MHC." in: Genomics, Vol. 31, Issue 2, pp. 215-22, 1997

Bilder

Produktdetails MICA ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für MICA Kit (ABIN411398) zurück nach oben
Bilder des Herstellers
ELISA image for MHC Class I Polypeptide-Related Sequence A (MICA) ELISA Kit (ABIN411398) Human MICA PicoKine ELISA Kit standard curve