anti-Human MICA Antikörper für Immunohistochemistry (Frozen Sections)

Recommended MICA Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
MHC Class I Polypeptide-Related Sequence A (MICA) Antikörper
  • Ica
  • mICA
  • FLJ60820
  • PERB11.1
  • MGC111087
  • MIC-A
  • RIKEN cDNA 1300017J02 gene
  • MHC class I polypeptide-related sequence A
  • 1300017J02Rik
  • MICA
Reaktivität
Human
179
14
11
10
7
7
2
Wirt
Maus
107
69
1
Klonalität
Monoklonal
Konjugat
Dieser MICA Antikörper ist unkonjugiert
16
8
7
6
5
5
5
5
5
5
5
4
1
1
Applikation
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
113
88
38
30
22
15
13
12
8
8
6
5
5
5
3
1
1
1
1
Hersteller
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Produktnummer ABIN180912
$ 500.50
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN180909 FACS IHC (fro) Biotin Mouse IgG2a Anmelden zum Anzeigen 6D4 6
1 ABIN180911 Func FACS IHC (fro) IP Mouse IgG2a Anmelden zum Anzeigen 6D4 6
1 ABIN601876 IHC (fro) FACS Biotin Mouse IgG2a Anmelden zum Anzeigen

General

Antigen MHC Class I Polypeptide-Related Sequence A (MICA) Antikörper
Reaktivität Human
(179), (14), (11), (10), (7), (7), (2)
Wirt Maus
(107), (69), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser MICA Antikörper ist unkonjugiert
(16), (8), (7), (6), (5), (5), (5), (5), (5), (5), (5), (4), (1), (1)
Applikation Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(113), (88), (38), (30), (22), (15), (13), (12), (8), (8), (6), (5), (5), (5), (3), (1), (1), (1), (1)
Pubmed 6 Publikationen vorhanden
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Produktdetails anti-MICA Antikörper

Target Details MICA Anwendungsinformationen Handhabung Referenzen für anti-MICA Antikörper (ABIN180912) Bilder
Spezifität This antibody is specific for nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB).
Kreuzreaktivität (Details) Also reacts to: MICB
Produktmerkmale Synonyms: MHC class I polypeptide-related sequence A, MIC-A, PERB11.1
Reinigung Affinity chromatography on Protein G
Immunogen MICA transfected C1R cells. Spleen cells from immunised RBF/DnJ mice were fused withcells of the P3 mouse myeloma cell line.
Klon 6D4
Isotyp IgG2a

Target Details MICA

Produktdetails anti-MICA Antikörper Anwendungsinformationen Handhabung Referenzen für anti-MICA Antikörper (ABIN180912) Bilder zurück nach oben
Antigen
Andere Bezeichnung MICA (MICA Antibody Abstract)
Hintergrund MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.Synonyms: MHC class I polypeptide-related sequence A, MIC-A, PERB11.1
Gen-ID 4276
UniProt Q29983
Forschungsgebiet Immunology, Innate Immunity, Adaptive Immunity
Pathways Activation of Innate immune Response, Transition Metal Ion Homeostasis

Anwendungsinformationen

Produktdetails anti-MICA Antikörper Target Details MICA Handhabung Referenzen für anti-MICA Antikörper (ABIN180912) Bilder zurück nach oben
Applikationshinweise Immunoprecipitation. Flow Cytometry: use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MICA Antikörper Target Details MICA Anwendungsinformationen Referenzen für anti-MICA Antikörper (ABIN180912) Bilder zurück nach oben
Konzentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Haltbarkeit 12 months

Referenzen für anti-MICA Antikörper (ABIN180912)

Produktdetails anti-MICA Antikörper Target Details MICA Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Das, Sugita, Brenner: "Mechanisms of Vdelta1 gammadelta T cell activation by microbial components." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6578-86, 2004

Jinushi, Takehara, Kanto, Tatsumi, Groh, Spies, Miyagi, Suzuki, Sasaki, Hayashi: "Critical role of MHC class I-related chain A and B expression on IFN-alpha-stimulated dendritic cells in NK cell activation: impairment in chronic hepatitis C virus infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 3, pp. 1249-56, 2003

Groh, Rhinehart, Randolph-Habecker, Topp, Riddell, Spies: "Costimulation of CD8alphabeta T cells by NKG2D via engagement by MIC induced on virus-infected cells." in: Nature immunology, Vol. 2, Issue 3, pp. 255-60, 2001

Bauer, Groh, Wu, Steinle, Phillips, Lanier, Spies: "Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 727-9, 1999

Groh, Rhinehart, Secrist, Bauer, Grabstein, Spies: "Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6879-84, 1999

Groh, Steinle, Bauer, Spies: "Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells." in: Science (New York, N.Y.), Vol. 279, Issue 5357, pp. 1737-40, 1998