anti-Fruchtfliege (Drosophila melanogaster) Heat Shock 60kDa Protein 1 (Chaperonin) Antikörper für ELISA

Recommended Heat Shock 60kDa Protein 1 (Chaperonin) Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antikörper
  • 12
  • BP5
  • CG12101
  • Cpn60
  • Dmel\\CG12101
  • Dmhsp60
  • G62
  • HSP60
  • HSP60A
  • Hsp60A
  • IEF16
  • Mmp-P1
  • SSP 7506
  • cpn60
  • d-hsp60
  • hsp60
  • hsp60A
  • l(1)10Ac
  • l(1)BP5
  • l(1)G8
  • l(1)HM21
  • l(1)L12
  • l(1)dp025
  • HSPD1
  • hld4
  • groel
  • hsp65
  • spg13
  • chaperonin
  • CPN60
  • MIF4
  • MNA2
  • mopA
  • groL
  • crpA
  • cb863
  • fa04a05
  • fb22d10
  • fi27b05
  • id:ibd2197
  • sb:cb144
  • wu:fa04a05
  • wu:fb22d10
  • wu:fi04a12
  • wu:fi27b05
  • GROEL
  • HLD4
  • HSP-60
  • HSP65
  • HuCHA60
  • SPG13
  • 60kDa
  • Hsp60
  • Hspd1-30p
  • Heat shock protein 60
  • heat shock 60kDa protein 1 (chaperonin)
  • chaperone ATPase HSP60
  • molecular chaperone GroEL
  • chaperonin GroEL
  • mitochondrial chaperonin
  • heat shock protein 60
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock protein 1 (chaperonin)
  • Hsp60
  • HSPD1
  • hspd1
  • HSP60
  • groEL
  • hsp60
  • groEl
  • Hspd1
Reaktivität
Huhn, Rind (Kuh), Hund, Fruchtfliege (Drosophila melanogaster), Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Pflanzen, Kaninchen, Ratte (Rattus), Schaf, Xenopus laevis
652
435
417
270
244
226
219
204
175
166
138
117
115
95
93
67
41
41
36
36
22
21
21
21
19
19
18
17
17
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Wirt
Maus
421
280
9
Klonalität
Monoklonal
Konjugat
Unkonjugiert
26
19
18
17
14
12
11
11
11
11
11
10
10
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
4
4
4
4
3
Applikation
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
622
288
287
277
266
262
197
187
84
13
12
10
8
7
5
2
2
1
1
Optionen
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Bilder

Produktnummer ABIN361784
$ 183.70
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN2481440 FACS IP IHC ELISA WB HRP Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481442 FACS IP IHC ELISA WB PerCP Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481431 FACS IP IHC ELISA WB Atto 594 Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481432 FACS IP IHC ELISA WB Atto 633 Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481436 FACS IP IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481441 FACS IP IHC ELISA WB PE-Atto 594 Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481444 FACS IP IHC ELISA WB Streptavidin Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481428 FACS IP IHC ELISA WB Atto 390 Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481429 FACS IP IHC ELISA WB Atto 488 Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481433 FACS IP IHC ELISA WB Atto 655 Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481434 FACS IP IHC ELISA WB Atto 680 Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481438 FACS IP IHC ELISA WB Biotin Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481443 FACS IP IHC ELISA WB PE Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481439 FACS IP IHC ELISA WB FITC Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481430 FACS IP IHC ELISA WB Atto 565 Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481435 FACS IP IHC ELISA WB Atto 700 Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN2481437 FACS IP IHC ELISA WB APC Mouse IgG1 Anmelden zum Anzeigen LK1 2
1 ABIN4320229 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 kappa AA 383-447 Anmelden zum Anzeigen LK1
1 ABIN4320230 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa AA 383-447 Anmelden zum Anzeigen LK1
1 ABIN4320223 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa AA 383-447 Anmelden zum Anzeigen LK1

General

Antigen Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antikörper
Reaktivität Huhn, Rind (Kuh), Hund, Fruchtfliege (Drosophila melanogaster), Meerschweinchen, Hamster, Human, Affe, Maus, Schwein, Pflanzen, Kaninchen, Ratte (Rattus), Schaf, Xenopus laevis
(652), (435), (417), (270), (244), (226), (219), (204), (175), (166), (138), (117), (115), (95), (93), (67), (41), (41), (36), (36), (22), (21), (21), (21), (19), (19), (18), (17), (17), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Wirt Maus
(421), (280), (9)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Unkonjugiert
(26), (19), (18), (17), (14), (12), (11), (11), (11), (11), (11), (10), (10), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (3)
Applikation Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(622), (288), (287), (277), (266), (262), (197), (187), (84), (13), (12), (10), (8), (7), (5), (2), (2), (1), (1)
Pubmed 17 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Spezifität Detects ~60 kDa.
Reinigung Protein G Purified
Immunogen Recombinant human HSP60
Klon LK1
Isotyp IgG1

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung HSP60 (HSPD1 Antibody Abstract)
Hintergrund In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
Gen-ID 3329
NCBI Accession NP_002147
UniProt P10809
Forschungsgebiet Heat Shock Proteins
Pathways Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikationshinweise
  • WB (1:20000)
  • IHC (1:100)
  • ICC/IF (1:100)
  • IP (1:200)
  • optimal dilutions for assays should be determined by the user.
Kommentare

0.05 μg/ml of SMC-110 was sufficient for detection of HSP60 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C

Referenzen

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Produkt verwendet in:

Dogan, Pujol, Maiti, Kukat, Wang, Hermans, Senft, Wibom, Rugarli, Trifunovic: "Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart." in: Cell metabolism, Vol. 19, Issue 3, pp. 458-69, 2014

Ahsan, Donnart, Nouri, Komatsu: "Tissue-specific defense and thermo-adaptive mechanisms of soybean seedlings under heat stress revealed by proteomic approach." in: Journal of proteome research, Vol. 9, Issue 8, pp. 4189-204, 2010

Ahsan, Komatsu: "Comparative analyses of the proteomes of leaves and flowers at various stages of development reveal organ-specific functional differentiation of proteins in soybean." in: Proteomics, Vol. 9, Issue 21, pp. 4889-907, 2009

Allgemeine Veröffentlichungen

Verda, Kim, Ikehara, Statkute, Bronesky, Petrenko, Oyama, He, Link, Vahanian, Burt: "Hematopoietic mixed chimerism derived from allogeneic embryonic stem cells prevents autoimmune diabetes mellitus in NOD mice." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 2, pp. 381-6, 2008

Lai, Zhuang, Zhang: "[Stability of implants placed in different bone types]" in: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology, Vol. 42, Issue 5, pp. 292-3, 2007

Lai, Liu, Ting, Yang, Huang, Wallace, Kaiser, Wang: "Regulation of IGF-I receptor signaling in diabetic cardiac muscle: dysregulation of cytosolic and mitochondria HSP60." in: American journal of physiology. Endocrinology and metabolism, Vol. 292, Issue 1, pp. E292-7, 2007

Deocaris, Kaul, Wadhwa: "On the brotherhood of the mitochondrial chaperones mortalin and heat shock protein 60." in: Cell stress & chaperones, Vol. 11, Issue 2, pp. 116-28, 2006

Gupta, Knowlton: "HSP60, Bax, apoptosis and the heart." in: Journal of cellular and molecular medicine, Vol. 9, Issue 1, pp. 51-8, 2005

Bason, Corrocher, Lunardi, Puccetti, Olivieri, Girelli, Navone, Beri, Millo, Margonato, Martinelli, Puccetti: "Interaction of antibodies against cytomegalovirus with heat-shock protein 60 in pathogenesis of atherosclerosis." in: Lancet (London, England), Vol. 362, Issue 9400, pp. 1971-7, 2003

Hartl, Hayer-Hartl: "Molecular chaperones in the cytosol: from nascent chain to folded protein." in: Science (New York, N.Y.), Vol. 295, Issue 5561, pp. 1852-8, 2002

Itoh, Komatsuda, Ohtani, Wakui, Imai, Sawada, Otaka, Ogura, Suzuki, Hamada: "Mammalian HSP60 is quickly sorted into the mitochondria under conditions of dehydration." in: European journal of biochemistry / FEBS, Vol. 269, Issue 23, pp. 5931-8, 2002

LaVerda, Kalayoglu, Byrne: "Chlamydial heat shock proteins and disease pathology: new paradigms for old problems?" in: Infectious diseases in obstetrics and gynecology, Vol. 7, Issue 1-2, pp. 64-71, 1999

Bukau, Horwich: "The Hsp70 and Hsp60 chaperone machines." in: Cell, Vol. 92, Issue 3, pp. 351-66, 1998

Neuer, Lam, Tiller, Kiesel, Witkin: "Humoral immune response to membrane components of Chlamydia trachomatis and expression of human 60 kDa heat shock protein in follicular fluid of in-vitro fertilization patients." in: Human reproduction (Oxford, England), Vol. 12, Issue 5, pp. 925-9, 1997

Hartl: "Molecular chaperones in cellular protein folding." in: Nature, Vol. 381, Issue 6583, pp. 571-9, 1996

Gao, Brosnan, Raine: "Experimental autoimmune encephalomyelitis. Qualitative and semiquantitative differences in heat shock protein 60 expression in the central nervous system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 7, pp. 3548-56, 1995

Jindal, Dudani, Singh, Harley, Gupta: "Primary structure of a human mitochondrial protein homologous to the bacterial and plant chaperonins and to the 65-kilodalton mycobacterial antigen." in: Molecular and cellular biology, Vol. 9, Issue 5, pp. 2279-83, 1989

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
 image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (ABIN361784) Hsp60 (LK 1), cell line mix.