anti-Alle Spezies Heat Shock 60kDa Protein 1 (Chaperonin) Antikörper für Immunoprecipitation

Recommended Heat Shock 60kDa Protein 1 (Chaperonin) Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antikörper
  • 12
  • BP5
  • CG12101
  • Cpn60
  • Dmel\\CG12101
  • Dmhsp60
  • G62
  • HSP60
  • HSP60A
  • Hsp60A
  • IEF16
  • Mmp-P1
  • SSP 7506
  • cpn60
  • d-hsp60
  • hsp60
  • hsp60A
  • l(1)10Ac
  • l(1)BP5
  • l(1)G8
  • l(1)HM21
  • l(1)L12
  • l(1)dp025
  • HSPD1
  • hld4
  • groel
  • hsp65
  • spg13
  • chaperonin
  • CPN60
  • MIF4
  • MNA2
  • mopA
  • groL
  • crpA
  • cb863
  • fa04a05
  • fb22d10
  • fi27b05
  • id:ibd2197
  • sb:cb144
  • wu:fa04a05
  • wu:fb22d10
  • wu:fi04a12
  • wu:fi27b05
  • GROEL
  • HLD4
  • HSP-60
  • HSP65
  • HuCHA60
  • SPG13
  • 60kDa
  • Hsp60
  • Hspd1-30p
  • Heat shock protein 60
  • heat shock 60kDa protein 1 (chaperonin)
  • chaperone ATPase HSP60
  • molecular chaperone GroEL
  • chaperonin GroEL
  • mitochondrial chaperonin
  • heat shock protein 60
  • heat shock 60kD protein 1 (chaperonin)
  • heat shock protein 1 (chaperonin)
  • Hsp60
  • HSPD1
  • hspd1
  • HSP60
  • groEL
  • hsp60
  • groEl
  • Hspd1
Reaktivität
Alle Spezies
653
436
418
271
245
227
220
205
176
167
139
117
116
96
94
67
42
41
36
36
22
21
21
21
19
19
18
17
17
4
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Wirt
Maus
421
280
9
Klonalität
Monoklonal
Konjugat
Unkonjugiert
26
19
18
17
14
12
11
11
11
11
11
10
10
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
4
4
4
4
3
Applikation
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
622
289
287
278
266
262
197
187
83
13
12
10
7
7
5
2
2
1
1
Hersteller
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Produktnummer ABIN5550932
$ 375.38
Zzgl. Versandkosten $45.00

General

Antigen Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antikörper
Reaktivität Alle Spezies
(653), (436), (418), (271), (245), (227), (220), (205), (176), (167), (139), (117), (116), (96), (94), (67), (42), (41), (36), (36), (22), (21), (21), (21), (19), (19), (18), (17), (17), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Wirt Maus
(421), (280), (9)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Unkonjugiert
(26), (19), (18), (17), (14), (12), (11), (11), (11), (11), (11), (10), (10), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (4), (4), (3)
Applikation Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(622), (289), (287), (278), (266), (262), (197), (187), (83), (13), (12), (10), (7), (7), (5), (2), (2), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Bilder
Spezifität This antibody recognizes a ~60 kDa protein, corresponding to the molecular mass of HSP60 on SDS-PAGE immunoblots (recognizes amino acids 383-447).
Kreuzreaktivität (Details) Species reactivity (tested):Human, Mouse, Rat, Bovine, Canine, Chicken, Drosophilia, Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep, Xenopus, Soybean.
Produktmerkmale Synonyms: HSP-60, HSPD1, Heat shock protein 60, Chaperonin 60, HuCHA60, GROEL, GroEL Homolog,CPN60, 60 kDa heat shock protein mitochondrial, 60 kDa chaperonin, Mitochondrialmatrix protein P1, P60 lymphocyte protein
Reinigung Purified
Immunogen Human Hsp60 produced through recombinant DNA methods in E. coli
Klon LK-1
Isotyp IgG1

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung HSP60 (HSPD1 Antibody Abstract)
Hintergrund In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. Hsp60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian Hsp60 (1-3). Whereas mammalian Hsp60 is localized within the mitochondria, plant Hsp60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that Hsp60 is present in so many different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, Hsp60 with its co-chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of Hsp60 and Hsp10 is their protective functions against infection and cellular stress. Hsp60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).Synonyms: 60 kDa chaperonin, 60 kDa heat shock protein mitochondrial, CPN60, Chaperonin 60, GROEL, GroEL Homolog, HSP-60, HSPD1, Heat shock protein 60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein
Gen-ID 3329
UniProt P10809
Forschungsgebiet Heat Shock Proteins
Pathways Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Bilder zurück nach oben
Applikationshinweise Western blot(10): 0.05 μg/mL. Immunoprecipitation (10). ELISA (11). Flow Cytometry (12). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Bilder zurück nach oben
Konzentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide and 50 % Glycerol
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.
Haltbarkeit 12 months