BLUeye Prestained Protein Ladder

Produktnummer: ABIN649019

Produktbeschreibung

Beschreibung: The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

Anwendungen

Buffer: Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). Bis-Tris 10% Tris-Glycine Bis-Tris 10% MES buffer 4~20% MOPS buffer

Lagerung: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar