GFP-Booster_Atto488

Antigen: Green Fluorescent Protein (GFP)

Applikation: Immunfluoreszenz (IF)

Produktnummer: ABIN509419

Produktbeschreibung

Beschreibung: GFP is a powerful tool to study protein localization and dynamics in living cells. However, the photo stability and the quantum efficiency of GFP is not sufficient for Super-Resolution Microscopy (e.g. 3D-SIM or STED) of fixed samples from cells expressing GFP-fusion proteins to visualize specific structures. Furthermore, many cell biological methods such as HCl treatment for BrdU-detection, the EdU-Click-iT™ treatment or heat denaturation for FISH lead to disruption of GFP signal. Here we offer our GFP-Booster _Atto488, a specific GFP-binding protein coupled to the fluorescent dye ATTO 488 (from ATTO-TEC) for reactivation, boosting and stabilization of GFP.

Anwendungen

Bestandteile: GFP-Trap® coupled to fluorescent dye ATTO 488

Konzentration: 1 mg/ml

Lagerung: Shipped at RT. Store at 4°C, do not freeze. Protect from light.

Forschungsgebiet: Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Enhancement of GFP signal with GFP-Booster after EdU-Click-iT™ treatment. EdU-Click-iT™ treament leads to disruption of GFP signal. GFP-Booster labels GFP fusion proteins and thus reactivates and boosts the fluorescence.