Argarase

Applikation: Klonierung (Clon)

Produktnummer: ABIN411761

Produktbeschreibung

Beschreibung: Agarase cleaves agarose to neoagaro-oligosaccharides, and is used for quantitative, gentle recovery of DNA from low melting point agarose. CONCENTRATION: 2 units/ μ l UNIT DEFINITION: One unit is defined as the amount of enzyme required to digest 100 μ l (approx. 100 mg) of molten 1% low melting point agarose to neoagaro-oligosaccharides in 1 hour at 45 o C. ENZYME STORAGE BUFFER: 40 mM Tris-HCl, pH 7.5, 50 mM NaCl, 50% glycerol (v/v).

Anwendungen

Applikationshinweise: Note: When the percentage of agarose in higher than 1%, the units of agarase have to be adjusted in proportion. When using Tris-borate gel electrophoresis buffer, a two-fold amount of agarase should be used, otherwise, the incubation time has to be prolonged. 4. Carefully mix the solution and incubate for 1 hour at 42-45 o C. 5. Add 0.1 volume of 3 M sodium acetate, pH 5.5, to the melten agarose solution and incubate 15 min on ice. 6. Centrifuge for 15 minutes at 2-8 o C to pellet the oligosacchrides. 7. Precipitate the nucleic acids from the supernatant with 3 volume of ice-cold ethanol as usual.

Buffer: 1 M Tris-HCl (pH 6.5), 10 mM EDTA

Lagerung: -20 o C

Beschränkungen: Nur für Forschungszwecke einsetzbar