Reagent EnzyBeads Trypsine 100

Applikation: Proteinverdauung (PD)

Produktnummer: ABIN410200

Produktbeschreibung

Produktmerkmale: EnzyBeads™ Trypsine offers a broad range of applications including protein identification and protein sequencing studies. Trypsin cleaves peptide bonds at the carboxylic side of lysine and arginine residues, creating peptides in the size range required for mass spectrometry analysis. The optimal pH of trypsin is between 7 and 9. EnzyBeads™ Trypsine are able to digest large as well as very small quantities of proteins (e.g few nanograms). A sample version of EnzyBeads™ Trypsine can be provided for testing (10 digestions).

Anwendungen

Protokoll: A protocol is provided with the product. This protocole is available in english or french.

Bestandteile: EnzyBeads Trypsine: 4 vials containing a suspension of magnetic beads with immobilized Trypsin (Trypsin from bovine pancreas, TPCK treated, the treatment with L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK) inhibits the chymotrypsin activity).

Benötigtes Material: Digestion Buffer advised: 15 mL of ammonium carbonate buffer (pH ~ 8; ~ 25 mM) for 1 tube of EnzyBeads Trypsine 25 digestions, Centrifuge microtubes or all microtubes compatible with the magnet, 5 uL to 200 uL adjustable pipettes, Disposables tips for pipettes, Deionised water (conductivity 18.2 Momega.cm at RT, 0.2 um filtered)

Lagerung: The reagent EnzyBeads Trypsine is stable 1 month at 2-8°C after opening, when free of contamination.

Beschränkungen: Nur für Forschungszwecke einsetzbar