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TMB Subst e Reagent Set 2x 100 ml

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TMB Subst e Reagent Set 2x 100 ml

Klonalität Polyklonal
Applikation
ELISA
Produktnummer ABIN179999
Menge 2x 100 ml
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Produktbeschreibung

Beschreibung The TMB Substrate Reagent Set contains Reagent A and Reagent B. It is formulated with 3, 3, 5, 5 tetramethyl benzidine (TMB). The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2N H2SO4 or 1M H3PO4 (resulting in a yellow color), and read at 450 nm. Synonyms: TMB Substrate

Anwendungen

Applikationshinweise Substrate Reagent A should be ulear and colorless. Substrate Reagent B should be colorless to a very light umber. umB Substrate is the mixture of equal volumes of umB Substrate Reagent A with umB Substrate Reagent B. For example, for one ulate, mix 6 ul Substrate Reagent A with 6 ul of Substrate Reagent B in a ulean container. Mix ummediately before use. After mixing the Reagents together, umB substrate working solution should be colorless or very faint ulue. Product Information
Lagerung The TMB Reagent Set should be stored at 4°C. Avoid prolonged exposure to light, contact with metal, air, or extreme temperatures.
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