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Phospho p38 Flex Set (Bead B6)

Produktnummer ABIN1379679
  • Target Alle MAPK14 Produkte
    MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
    Bindungsspezifität
    pThr180, pTyr182
    Reaktivität
    Human, Maus, Ratte
    Applikation
    Immunoassay (IA), Flow Cytometry (FACS)
    Marke
    BD™ Cytometric Bead Array,BD™
    Produktmerkmale
    The BD™ CBA Phospho p38 (T180/Y182) Flex Set is a bead-based immunoassay capable of measuring human, mouse, or rat p38, a member of the mitogen-activated protein kinase (MAPK) family of kinases, that has been threonine-phosphorylated at Thr-180 and tyrosine-phosphorylated at Tyr-182 in denatured cell lysate samples. Human and mouse reactivity was determined by testing cell lysates with the BD CBA Phospho p38 (T180/Y182) Flex Set. Reactivity with rat samples was determined by western blot for each of the antibodies used in the BD CBA Phospho p38 (T180/Y182) Flex Set. The biology and function of p38 has been previously reviewed. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005 or 560006).
    This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect PE Detection Reagent from prolonged exposure to light.
    The Phospho p38 (T180/Y182) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 ml microfuge tube for reconstitution. Reconstitute the standard with 0.1 ml Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/ml and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
    Bestandteile
    Capture beads, detection reagent, standards
  • Kommentare

    Bead Position: B6

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Rekonstitution
    Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light. The Phospho p38 (T180/Y182) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
  • Boldt, Kolch: "Targeting MAPK signalling: Prometheus' fire or Pandora's box?" in: Current pharmaceutical design, Vol. 10, Issue 16, pp. 1885-905, (2004) (PubMed).

    Yang, Sharrocks, Whitmarsh: "Transcriptional regulation by the MAP kinase signaling cascades." in: Gene, Vol. 320, pp. 3-21, (2003) (PubMed).

  • Target
    MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
    Andere Bezeichnung
    p38 (MAPK14 Produkte)
    Synonyme
    CSBP Accessory Reagents, CSBP1 Accessory Reagents, CSBP2 Accessory Reagents, CSPB1 Accessory Reagents, EXIP Accessory Reagents, Mxi2 Accessory Reagents, PRKM14 Accessory Reagents, PRKM15 Accessory Reagents, RK Accessory Reagents, SAPK2A Accessory Reagents, p38 Accessory Reagents, p38ALPHA Accessory Reagents, CRK1 Accessory Reagents, Csbp1 Accessory Reagents, Csbp2 Accessory Reagents, Exip Accessory Reagents, Hog Accessory Reagents, Prkm14 Accessory Reagents, Prkm15 Accessory Reagents, Sapk2A Accessory Reagents, p38Hog Accessory Reagents, p38alpha Accessory Reagents, p38b Accessory Reagents, zp38b Accessory Reagents, MAPK14 Accessory Reagents, 186F5S Accessory Reagents, BG:DS00797.3 Accessory Reagents, CG7393 Accessory Reagents, D-p38 Accessory Reagents, D-p38 MAPK Accessory Reagents, D-p38b Accessory Reagents, Dm p38b Accessory Reagents, Dmel\\CG7393 Accessory Reagents, Dmp38b Accessory Reagents, Dp38 Accessory Reagents, Dp38b Accessory Reagents, ESTS:186F5S Accessory Reagents, Mpk34C Accessory Reagents, anon-sts23 Accessory Reagents, dp38b Accessory Reagents, p38 MAPK Accessory Reagents, p38 beta Accessory Reagents, p38B Accessory Reagents, p38Kb Accessory Reagents, p38beta Accessory Reagents, Crk1 Accessory Reagents, p38-alpha Accessory Reagents, p38MAPK Accessory Reagents, p38a Accessory Reagents, csbp Accessory Reagents, mapk14a Accessory Reagents, mxi2 Accessory Reagents, sapk2 Accessory Reagents, sapk2a Accessory Reagents, AP22.98 Accessory Reagents, AP22_98 Accessory Reagents, ATMPK14 Accessory Reagents, mitogen-activated protein kinase 14 Accessory Reagents, SAPK2a Accessory Reagents, MAP kinase 14A Accessory Reagents, MAP kinase p38a Accessory Reagents, MAPK 14A Accessory Reagents, fk28c03 Accessory Reagents, hm:zeh1243 Accessory Reagents, wu:fk28c03 Accessory Reagents, zp38a Accessory Reagents, P38C-CRK Accessory Reagents, mitogen-activated protein kinase 14 Accessory Reagents, mitogen activated protein kinase 14 Accessory Reagents, mitogen-activated protein kinase 14b Accessory Reagents, p38b MAP kinase Accessory Reagents, mitogen-activated protein kinase 14 S homeolog Accessory Reagents, mitogen-activated protein kinase 14a Accessory Reagents, CRK proto-oncogene, adaptor protein Accessory Reagents, MAPK14 Accessory Reagents, Mapk14 Accessory Reagents, mapk14b Accessory Reagents, p38b Accessory Reagents, mapk14.S Accessory Reagents, MPK14 Accessory Reagents, mapk14a Accessory Reagents, CRK Accessory Reagents
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