Blocked agarose beads

Details zu Produkt Nr. ABIN1082210, Anbieter: Anmelden zum Anzeigen
Applikation
Affinity Measurement (AM), Chromatin Immunoprecipitation (ChIP), Enzyme Activity Assay (EAA), Immunoprecipitation (IP), Mass Spectrometry (MS), Protein Complex Immunoprecipitation (Co-IP), Pull-Down Assay (Pull-Down), Purification (Purif), Separation (Sep)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck Blocked agarose beads as binding control for Dnmt1-Trap A, GFP-Trap A, RFP-Trap A
Produktmerkmale Antibodies - extremely powerful tools in biomedical research - are large complex molecules (~ 150 kDa) consisting of two heavy and two light chains. Due to their complex structure, the use of antibodies is often limited and hindered by batch-to-batch variations.

Camelidae (camels, dromedaries, llamas and alpacas) possess functional antibodies devoid of light chains, so-called heavy chain antibodies (hcAbs). hcAbs recognize and bind their antigens via a single variable domain (VHH). These VHH domains are the smallest intact antigen binding fragments (~ 13 kDa).

Nano-Traps are based on single domain antibody fragments (VHHs) derived from alpaca.
Bestandteile Blocked agarose beads
Kommentare

Bead size ~ 80 µm

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 20% EtOH
Handhabung Do not freeze.
Lagerung 4 °C
Haltbarkeit 12 months
Bilder des Herstellers
Immunoprecipitation (IP) image for Blocked agarose beads (ABIN1082210) Tested application: IP
Western Blotting (WB) image for Blocked agarose beads (ABIN1082210) Tested application: WB
Produkt verwendet in: Berk, Maitra, Dawdy, Shabanowitz, Hunt, Wilson: "O-Linked ?-N-acetylglucosamine (O-GlcNAc) regulates emerin binding to barrier to autointegration factor (BAF) in a chromatin- and lamin B-enriched "niche"." in: The Journal of biological chemistry, Vol. 288, Issue 42, pp. 30192-209, 2013 (PubMed).