rBmKTX

Details zu Produkt Nr. ABIN1001635, Anbieter: Anmelden zum Anzeigen
Reaktivität
Scorpion
Wirt
Escherichia coli (E. coli)
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Sequenz VGINVKCKHS GQCLKPCKDA GMRFGKCING KCDCTPK.
Spezifität Voltage-gated channel (Kv1.3) blocker. rBmKTX blocks Kv1.3 channels with IC50 of 019 nM.
Produktmerkmale Source: Mesobuthus Martensii (Buthus martensii).
Molecular formula: C169H274N52O47S7.
Disulfide bonds between Cys7-Cys27, Cys13-Cys32, and Cys17-Cys34.
Reinheit > 98 %
Hintergrund BmKTX inhibits Kv1.3 channels heterologously expressed in HEK-293 cells. Kv1.3 channel currents, before and after application of 100pM BmKTX. Kv1.3 channel currents were elicited by depolarizing the voltage steps of 200 ms from the holding potential -80 mV to +50 mV.
Molekulargewicht 3962
Applikationshinweise 0.1-1nM
Kommentare

K+ channel toxin -KTx 3.6

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Concentration after reconstitution: Dissolving 50g in 1.262ml will provide a stock solution of 10 M.
Handhabung Soluble in Water.
Bilder des Herstellers
 image for rBmKTX (ABIN1001635) Kv1.3 channel currents, before and after application of 100pM BmKTX. Kv1.3 channel cu...
Produkt verwendet in: Renisio, Romi-Lebrun, Blanc, Bornet, Nakajima, Darbon: "Solution structure of BmKTX, a K+ blocker toxin from the Chinese scorpion Buthus Martensi." in: Proteins, Vol. 38, Issue 1, pp. 70-8, 2000 (PubMed).

Dai, Wu, Gu, Lan, Ling, Chi: "Genomic organization of three novel toxins from the scorpion Buthus martensi Karsch that are active on potassium channels." in: The Biochemical journal, Vol. 346 Pt 3, pp. 805-9, 2000 (PubMed).

Romi-Lebrun, Lebrun, Martin-Eauclaire, Ishiguro, Escoubas, Wu, Hisada, Pongs, Nakajima: "Purification, characterization, and synthesis of three novel toxins from the Chinese scorpion Buthus martensi, which act on K+ channels." in: Biochemistry, Vol. 36, Issue 44, pp. 13473-82, 1997 (PubMed).