rScyllatoxin

Details zu Produkt Nr. ABIN1001609, Anbieter: Anmelden zum Anzeigen
Reaktivität
Scorpion
Wirt
Escherichia coli (E. coli)
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Sequenz AFCNLRMCQL SCRSLGLLGK CIGDKCECVK H.
Spezifität Scyllatoxin inhibits small conductance Ca2+-activated K+ channels (SK) 1-3.
Produktmerkmale Source: Leiurus quinquestriatus hebraeus (Yellow scorpion).
Molecular formula: C142H237N45O39S7.
Disulfide bonds between Cys3-Cys21, Cys8-Cys26, and Cys12-Cys28.
Reinheit > 98 %
Hintergrund Scyllatoxin inhibits SKCa3 channels heterologously expressed in HEK-293 cells. SKCa3 channel currents, before and after application of 100nM rScyllatoxin. The SKCa currents were elicited by 1µM internal calcium and 200 ms voltage ramps from -140 to +40 mV at the holding potential of 0 mV.
Molekulargewicht 3424
Applikationshinweise 20-200 nM
Kommentare

K+ channel toxin -KTx 5.1, Leiurotoxin I, LeTxI, ScyTx

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Concentration after reconstitution: Dissolving 50g in 1.460ml will provide a stock solution of 10 M.
Handhabung Soluble in Water.
Bilder des Herstellers
 image for rScyllatoxin (ABIN1001609) SKCa3 channel currents, before and after application of 100nM rScyllatoxin. The SKCa ...
Produkt verwendet in: Rodríguez de la Vega, Possani: "Current views on scorpion toxins specific for K+-channels." in: Toxicon : official journal of the International Society on Toxinology, Vol. 43, Issue 8, pp. 865-75, 2004 (PubMed).

Pedarzani, Kulik, Muller, Ballanyi, Stocker: "Molecular determinants of Ca2+-dependent K+ channel function in rat dorsal vagal neurones." in: The Journal of physiology, Vol. 527 Pt 2, pp. 283-90, 2000 (PubMed).

Strøbaek, Jørgensen, Christophersen, Ahring, Olesen: "Pharmacological characterization of small-conductance Ca(2+)-activated K(+) channels stably expressed in HEK 293 cells." in: British journal of pharmacology, Vol. 129, Issue 5, pp. 991-9, 2000 (PubMed).

Wadsworth, Doorty, Strong: "Comparable 30-kDa apamin binding polypeptides may fulfill equivalent roles within putative subtypes of small conductance Ca(2+)-activated K+ channels." in: The Journal of biological chemistry, Vol. 269, Issue 27, pp. 18053-61, 1994 (PubMed).

Grissmer, Lewis, Cahalan: "Ca(2+)-activated K+ channels in human leukemic T cells." in: The Journal of general physiology, Vol. 99, Issue 1, pp. 63-84, 1992 (PubMed).

Auguste, Hugues, Mourre, Moinier, Tartar, Lazdunski: "Scyllatoxin, a blocker of Ca(2+)-activated K+ channels: structure-function relationships and brain localization of the binding sites." in: Biochemistry, Vol. 31, Issue 3, pp. 648-54, 1992 (PubMed).

Goh, Kelly, Pennefather, Chicchi, Cascieri, Garcia, Kaczorowski: "Effect of charybdotoxin and leiurotoxin I on potassium currents in bullfrog sympathetic ganglion and hippocampal neurons." in: Brain research, Vol. 591, Issue 1, pp. 165-70, 1992 (PubMed).

Auguste, Hugues, Gravé, Gesquière, Maes, Tartar, Romey, Schweitz, Lazdunski: "Leiurotoxin I (scyllatoxin), a peptide ligand for Ca2(+)-activated K+ channels. Chemical synthesis, radiolabeling, and receptor characterization." in: The Journal of biological chemistry, Vol. 265, Issue 8, pp. 4753-9, 1990 (PubMed).

Chicchi, Gimenez-Gallego, Ber, Garcia, Winquist, Cascieri: "Purification and characterization of a unique, potent inhibitor of apamin binding from Leiurus quinquestriatus hebraeus venom." in: The Journal of biological chemistry, Vol. 263, Issue 21, pp. 10192-7, 1988 (PubMed).