rlberiotoxin (IbTx)

Details zu Produkt Nr. ABIN1001608, Anbieter: Anmelden zum Anzeigen
Reaktivität
Scorpion
Wirt
Escherichia coli (E. coli)
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Sequenz QFTDVDCSVS KECWSVCKDL FGVDRGKCMG KKCRCYQ.
Spezifität Iberiotoxin is a potent selective blocker of the high conductance Ca2+-activated K+ channels (maxi-K).
Produktmerkmale Source: Mesobuthus tamulus (Eastern Indian scorpion).
Molecular formula: C179H277N51O55S7.
Disulfide bonds between Cys7-Cys28, Cys13-Cys33, and Cys17-Cys35.
Reinheit > 98 %
Hintergrund Inhibition of BKCa channels heterologously expressed in HEK293 cells by Iberiotoxin. mSlo channel currents, before and during application of 100nM rIberiotoxin. The mSlo currents were activated by a 50 ms voltage step to +100 mV from a prepulse of -180 mV with intracellular 10 M Ca2+.
Molekulargewicht 4248
Applikationshinweise 50-100 nM
Kommentare

K+ channel toxin -KTx 1.3

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Concentration after reconstitution: Dissolving 50 g in 1.175ml will provide a stock solution of 10 M.
Handhabung Soluble in Water.
Bilder des Herstellers
 image for rlberiotoxin (IbTx) (ABIN1001608) mSlo channel currents, before and during application of 100nM rIberiotoxin. The mSlo ...
Allgemeine Veröffentlichungen Meera, Wallner, Toro: "A neuronal beta subunit (KCNMB4) makes the large conductance, voltage- and Ca2+-activated K+ channel resistant to charybdotoxin and iberiotoxin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 10, pp. 5562-7, 2000 (PubMed).

Produkt verwendet in: Koschak, Koch, Liu, Kaczorowski, Reinhart, Garcia, Knaus: "[125I]Iberiotoxin-D19Y/Y36F, the first selective, high specific activity radioligand for high-conductance calcium-activated potassium channels." in: Biochemistry, Vol. 36, Issue 7, pp. 1943-52, 1997 (PubMed).

Galvez, Gimenez-Gallego, Reuben, Roy-Contancin, Feigenbaum, Kaczorowski, Garcia: "Purification and characterization of a unique, potent, peptidyl probe for the high conductance calcium-activated potassium channel from venom of the scorpion Buthus tamulus." in: The Journal of biological chemistry, Vol. 265, Issue 19, pp. 11083-90, 1990 (PubMed).