anti-Mdc1 (MDC1) Antikörper

The protein encoded by MDC1 contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence.

Alle Antikörper anzeigen Gen GeneID UniProt
MDC1 240087 Q5PSV9
MDC1 309595 Q5U2M8
MDC1 9656 Q14676
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Top anti-MDC1 Antikörper auf antikoerper-online.de

Showing 10 out of 164 products:

Katalog Nr. Reaktivität Wirt Konjugat Applikation Bilder Menge Anbieter Lieferzeit Preis Details
Human Kaninchen Unkonjugiert IHC, ELISA Immunohistochemistry analysis of paraffin-embedded human brain, using MDC1 (Phospho-Ser513) Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Anmelden zum Anzeigen 6 bis 8 Tage
342,86 €
Details
Hund Kaninchen Unkonjugiert WB WB Suggested Anti-MDC1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:12500 Positive Control: HepG2 cell lysate MDC1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells 100 μL Anmelden zum Anzeigen 6 bis 8 Tage
358,51 €
Details
Human Kaninchen Unkonjugiert IHC (p), IP, IHC, WB Detection of Human MDC1 by Western Blot and Immunoprecipitation. Samples: A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) Affinity purified rabbit anti-MDC1 antibody ABIN151860 used at the indicated concentrations for WB. B) Affinity purified rabbit anti-MDC1 antibodies ABIN151860 and ABIN151861 used at 3.3 mcg/mg lysate for IP followed by WB using ABIN151859 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times less than 5 min. Immunohistochemistry-Paraffin: MDC1 Antibody [ABIN151860] - Human ovarian carcinoma. 100 μL Anmelden zum Anzeigen 2 bis 4 Tage
371,00 €
Details
Human Kaninchen Unkonjugiert ICC, IF, PLA, WB Detection of Human MDC1 by Western Blot. Samples: A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) Affinity purified rabbit anti-MDC1 antibody ABIN151859 used at the indicated concentrations for WB. B) Affinity purified rabbit anti-MDC1 antibodies ABIN151860 and ABIN151861 used at 3.3 mcg/mg lysate for IP followed by WB using ABIN151458 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times less than 5 min. Proximity Ligation Assay: MDC1 Antibody [ABIN151859] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMC1 (NBP2-14858) and rabbit anti-human MDC1 (ABIN151859). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification. 100 μL Anmelden zum Anzeigen 2 bis 4 Tage
371,00 €
Details
Human Kaninchen Unkonjugiert ICC, IF, IHC (p), IP, IHC, WB Detection of Human MDC1 by Western Blot and Immunoprecipitation. Samples: A) Nuclear extract (50 mcg) from HeLa cells. B) Whole cell lysate from HEK 293 cells. Antibodies: A) Affinity purified rabbit anti-MDC1 antibody ABIN151851 used at the indicated concentrations for WB. B) Affinity purified rabbit anti-MDC1 antibodies ABIN151860 and ABIN151861 used at 3.3 mcg/mg lysate for IP followed by WB using ABIN151860 at 0.1 mcg/ml. Detection: Chemiluminescence with exposure times less than 5 min. Immunohistochemistry-Paraffin: MDC1 Antibody [ABIN151861] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1 0.1 mL Anmelden zum Anzeigen 2 bis 4 Tage
371,00 €
Details
Human Kaninchen Unkonjugiert ICC, IF, IHC, IHC (p) Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860] - Staining of human colon shows strong nuclear and membranous positivity in glandular cells. Immunocytochemistry/Immunofluorescence: MDC1 Antibody [NBP1-84860] Staining of human cell line U-251 MG shows positivity in nucleus but not nucleoli & focal adhesions. 0.1 mL Anmelden zum Anzeigen 5 bis 8 Tage
335,00 €
Details
Schimpanse Maus Unkonjugiert ICC, IF, WB Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 . Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X. Western Blot analysis of Human 293Trap cell lysates showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 . Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody  at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP: IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa. 100 μg Anmelden zum Anzeigen 6 bis 8 Tage
386,72 €
Details
Human Kaninchen Unkonjugiert IF (p), IHC (p) Antigen: 0.2 µg/100 µL  Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;  Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000;  TMB staining;  Read the data in MicroplateReader by 450 Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti NFBD1/MDC1 Polyclonal Antibody, Unconjugated (ABIN739740) at 1:200 followed by conjugation to the secondary antibody and DAB staining 100 μL Anmelden zum Anzeigen 9 bis 12 Tage
304,36 €
Details
Human Kaninchen Unkonjugiert IHC (p), WB IHC-P Image Immunohistochemical analysis of paraffin-embedded 59T xenograft, using MDC1, antibody at 1:500 dilution. WB Image Sample (30 ug of whole cell lysate) A: HeLa nucleus 5% SDS PAGE antibody diluted at 1:1000 100 μL Anmelden zum Anzeigen 5 bis 7 Tage
357,24 €
Details
Human Kaninchen Unkonjugiert EIA, WB   0.1 mg Anmelden zum Anzeigen 7 bis 9 Tage
495,00 €
Details

MDC1 Antikörper nach Reaktivität, Anwendung, Klonalität and Konjugat

Attribute Applikationen Wirt Klonalität Konjugat
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Am meisten referenzierte anti-MDC1 Antikörper

  1. Human Polyclonal MDC1 Primary Antibody für ICC, IF - ABIN151859 : Shi, Ma, Willers, Akhtar, Scott, Zhang, Powell, Zhang: Disassembly of MDC1 foci is controlled by ubiquitin-proteasome-dependent degradation. in The Journal of biological chemistry 2008 (PubMed)
    Zeige alle 16 Referenzen für 151859

  2. Chimpanzee Monoclonal MDC1 Primary Antibody für ICC, IF - ABIN863114 : Lou, Chen, Asaithamby, Minter-Dykhouse, Chen, Chen: MDC1 regulates DNA-PK autophosphorylation in response to DNA damage. in The Journal of biological chemistry 2004 (PubMed)
    Zeige alle 4 Referenzen für 863114

  3. Human Polyclonal MDC1 Primary Antibody für IHC, ELISA - ABIN1532151 : Shiina, Ota, Shimizu, Katsuyama, Hashimoto, Takasu, Anzai, Kulski, Kikkawa, Naruse, Kimura, Yanagiya, Watanabe, Hosomichi, Kohara, Iwamoto, Umehara, Meyer, Wanner, Sano, Macquin, Ikeo, Tokunaga et al.: Rapid evolution of major histocompatibility complex class I genes in primates generates new disease alleles in humans via hitchhiking diversity. ... in Genetics 2006 (PubMed)
    Zeige alle 2 Referenzen für 1532151

  4. Human Polyclonal MDC1 Primary Antibody für ICC, IF - ABIN4333285 : Mastrocola, Kim, Trinh, Rodenkirch, Tibbetts: The RNA-binding protein fused in sarcoma (FUS) functions downstream of poly(ADP-ribose) polymerase (PARP) in response to DNA damage. in The Journal of biological chemistry 2013 (PubMed)

  5. Human Polyclonal MDC1 Primary Antibody für ICC, IF - ABIN151861 : Mallette, Mattiroli, Cui, Young, Hendzel, Mer, Sixma, Richard: RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites. in The EMBO journal 2012 (PubMed)

  6. Human Polyclonal MDC1 Primary Antibody für IHC (p), IP - ABIN151860 : Floyd, Pacold, Huang, Clarke, Lam, Cannell, Bryson, Rameseder, Lee, Blake, Fydrych, Ho, Greenberger, Chen, Maffa, Del Rosario, Root, Carpenter, Hahn, Sabatini, Chen, White, Bradner, Yaffe: The bromodomain protein Brd4 insulates chromatin from DNA damage signalling. in Nature 2013 (PubMed)

Weitere Antikörper gegen MDC1 Interaktionspartner

Mouse (Murine) Mdc1 (MDC1) Interaktionspartner

  1. MDC1 plays a fundamentally significant role in maintenance of genomic stability through a DNA damage response-independent pathway.

  2. MDC1 silencing enhances the radiosensitivity of human nasopharyngeal cancer CNE1 cells and results in xenograft tumor growth inhibition.

  3. oligomerization of MDC1 plays an important role in DDR (zeige DDR1 Antikörper) and help understand the formation of proteins complexes at the sites of DNA damage.

  4. structural insight into MDC1-CHK2 (zeige CHEK2 Antikörper) interaction

  5. the DNA damage response pathway centered on MDC1 triggers epigenetic silencing of sex chromosomes in germ cells

  6. Data show that loss of Mof (zeige KAT8 Antikörper) leads to reduction of histone H4 (zeige HIST1H4H Antikörper) K16 (zeige KRT16 Antikörper) acetylation, cell cycle arrest, chromosome aberration, defects in DNA damage repair, and complete loss of Mdc1 response to DNA damage.

  7. Data show that ATM (zeige ATM Antikörper)-dependent resection at a subset of DSBs leads to ATR (zeige ATR Antikörper)-dependent Chk1 (zeige CHEK1 Antikörper) activation, and that 53BP1 (zeige TP53BP1 Antikörper)(-/-) and MDC1(-/-) cells manifest a checkpoint defect at high radiation doses.

  8. identification and description of a functional homologue of human MDC1/ NFBD1; in response to ionizing radiation it forms foci that co-localize with the MRE11 (zeige MRE11A Antikörper)-RAD50 (zeige RAD50 Antikörper)-NBS1 (zeige NBN Antikörper) (MRN) complex and factors such as gammaH2AX (zeige H2AFX Antikörper) and 53BP1 (zeige TP53BP1 Antikörper)

  9. MDC1, as a signal amplifier of the ATM (zeige ATM Antikörper) pathway, is vital in controlling proper DNA damage response and maintaining genomic stability.

  10. MDC1 knockdown affected the formation of telomere dysfunction-induced foci.

Human Mdc1 (MDC1) Interaktionspartner

  1. the opposing activities of RNF4 (zeige RNF4 Antikörper) and ataxin-3 (zeige ATXN3 Antikörper) consolidate robust MDC1-dependent signaling and repair ofDNA double-strand break.

  2. NFBD1 protein is overexpressed in NPC (zeige NPC1 Antikörper) tissues and that silencing NFBD1 can inhibit cell growth, induce apoptosis, increase the production of intracellular ROS (zeige ROS1 Antikörper). NFBD1 knockdown also inhibits the tumorigenicity of NPC (zeige NPC1 Antikörper) cells in vivo.

  3. NFBD1 knockdown improves the chemosensitivity of NPC (zeige NPC1 Antikörper) cells by inhibiting cell growth and promoting apoptosis through the impairment of DNA damage repair, suggesting NFBD1 as a novel therapeutic target for NPC (zeige NPC1 Antikörper).

  4. knockdown of MCM2 or MCM6 could significantly inhibit foci forming of MDC1 in TE-1 nuclei in response to bleomycin-induced DNA damage (p < 0.001). This study indicates the direct interaction between MDC1 and MCMs in TE-1 nuclei.

  5. The interaction of MDC1 with RNF8 (zeige RNF8 Antikörper), but not with ATM (zeige ATM Antikörper) requires WRAP53beta, suggesting that WRAP53beta facilitates the former interaction without altering phosphorylation of MDC1 by ATM (zeige ATM Antikörper).

  6. MDC1 recruits TNKS1 (zeige TNKS Antikörper) and TNKS2 (zeige TNKS2 Antikörper) to DNA lesions.

  7. MDC1 silencing enhances the radiosensitivity of human nasopharyngeal cancer CNE1 cells and results in xenograft tumor growth inhibition.

  8. we generated two HEP (zeige EPHB6 Antikörper)-2 cell lines with a stable knockdown of MDC1 or 53BP1 (zeige TP53BP1 Antikörper) with short hairpin RNA (shRNA), respectively, and investigated the effect of MDC1 and 53BP1 (zeige TP53BP1 Antikörper) on cell radiosensitivity

  9. During replicative senescence and stress-induced premature senescence, MDC1 is downregulated by upregulating miR (zeige MLXIP Antikörper)-22.

  10. we have identified a novel antisense lncRNA MDC1-AS, which may participate in bladder cancer through up-regulation of its antisense tumor-suppressing gene MDC1

MDC1 Antigen-Profil

Protein Überblick

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Genbezeichner und Symbole assoziert mit anti-Mdc1 (MDC1) Antikörper

  • mediator of DNA damage checkpoint 1 (Mdc1) Antikörper
  • mediator of DNA-damage checkpoint 1 (MDC1) Antikörper
  • 6820401C03 Antikörper
  • AA413496 Antikörper
  • mKIAA0170 Antikörper
  • NFBD1 Antikörper

Bezeichner auf Proteinebene für anti-Mdc1 (MDC1) Antikörper

mediation of DNA damage checkpoint 1 , mediator of DNA damage checkpoint protein 1 , homologue to Drosophila photoreceptor protein calphotin , mediator of DNA damage checkpoint 1 , nuclear factor with BRCT domains 1

GENE ID SPEZIES
240087 Mus musculus
309595 Rattus norvegicus
9656 Homo sapiens
474829 Canis lupus familiaris
100144453 Sus scrofa
511168 Bos taurus
471959 Pan troglodytes
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